摘要
目的观察中药活性成分姜酚对类风湿关节炎(RA)模型大鼠血清白细胞介素(IL)-17、IL-23的影响,探讨其作用机制。方法 40只Wistar大鼠随机分为正常组、模型组、姜酚组和雷公藤组。采用胶原诱导制备RA模型,于造模第7日开始,姜酚组给予姜酚灌胃,雷公藤组给予雷公藤多苷灌胃,正常组和模型组给予等量生理盐水灌胃。治疗4周后采血并处死大鼠,镜下观察大鼠关节滑膜病理,ELISA检测大鼠血清IL-17、IL-23的表达。结果病理观察结果显示,与模型组比较,姜酚组关节滑膜组织血管新生及滑膜增生减轻,炎细胞浸润、水肿改善。模型组IL-17、IL-23表达明显高于正常组(P<0.01);与模型组比较,姜酚组和雷公藤组均能下调IL-17、IL-23表达(P<0.05,P<0.01),姜酚组优于雷公藤组(P<0.05)。结论姜酚通过下调RA大鼠血清IL-17、IL-23的表达起到一定的治疗作用。
Objective To observe the effects of Chinese medicine ingredient ginger phenol on expressions of IL-17 and IL-23 in collagen-induced arthritis （CIA） rats; To investigate the role and mechanism of ginger phenol in CIA rats. Methods Forty female Wistar rats were randomly divided into 4 groups： blank group, model control group, ginger phenol treatment group and tripterygium glucosides control group. After CIA was established in rats successfully, ginger phenol treatment group and tripterygium glycosides control group were treated with ginger phenol and tripterygium glycosides by gavage, while the control group and model control group were fed with the same amount of saline. Four weeks after the treatment, the rats were collected serum and sacrificed. The pathological of CIA joint synovial was observed by electron microscope. The expressions of IL-17 and IL-23 in serum were detected by ELISA. Results The hyperplasia of synovial tissue and angiogenesis was reduced in gingerol phenol treatment group. Infiltration of inflammatory cells and edema was improved. IL-17 and IL-23 expressions of in serum of CIA rats were significantly higher than those of the blank group （P^0.01）. Ginger phenol and tripterygium glycosides down-regulated the expressions of IL-23 and IL-17 （P〈0.05, P〈0.01）. Ginger phenol group was better than that of tripterygium glycosides control group （P〈0.05）. Conclusion Chinese medicine ingredient ginger phenol can reduce expressions IL-17 and IL-23 in serum of CIA rats.
出处
《中国中医药信息杂志》
CAS
CSCD
2014年第9期43-45,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家中医药管理局重点建设专科项目(2012年)
贵州省中医药民族药重点学科项目(2011年)
贵州省科技创新人才团队项目(黔科合人才团队[2013]4033)
