摘要
目的评价补阳还五汤及其精简方对脑缺血损伤的保护作用。方法体内实验:SD大鼠按体质量随机分为假手术组、局灶性脑缺血(MCAO)模型组、补阳还五汤组和补阳还五汤精简方组,每组10只,观察各组大鼠体质量、脑梗死面积和脑含水量等指标的变化。体外实验:采用过氧化氢(H2O2)对大鼠肾上腺嗜铬细胞瘤细胞(PC12细胞)致损,建立体外氧化应激细胞模型,将PC12细胞分为正常组、空白对照组、补阳还五汤组和补阳还五汤精简方不同浓度组(0.1、0.2、0.5、1.0、2.0、3.5 mg/mL)及模型组,MTT法考察补阳还五汤及其精简方对模型细胞的保护作用。结果体内实验表明,补阳还五汤及其精简方给药7 d后,均能恢复MCAO模型大鼠的体质量、降低脑梗死面积、减轻脑组织水肿,与模型组比较差异有统计学意义(P<0.05),两方组间比较差异无统计学意义(P>0.05);体外实验表明,补阳还五汤及其精简方与细胞作用2 h即能发挥保护作用,对H2O2所致的氧化应激损伤有明显的保护作用,与模型组比较差异有统计学意义(P<0.05),细胞存活率升高,同一浓度两方组间比较差异无统计学意义(P>0.05)。结论体内外实验均表明,补阳还五汤精简方在抗脑缺血损伤方面功效显著,且药效不亚于补阳还五汤,在保证药效的前提下大大节约了中药资源。
Objective To investigate the preventive effects of Buyang Huanwu Decoction （BYHWD） and its recipe composition （BYJJF） in focal ischemic brain injury condition in vivo/in vitro. Methods In vivo studies, SD rats were divided into sham-operation group, MCAO group, BYHWD group and BYJJF group based on rat weight, 10 rats in each group. The body weight, infarct area and brain water contents were determined. In vitro studies, H202 was used to damage PC 12 cells, and the vitro oxidative stress cell model was established. PC12 cells were divided into normal group, blank control group, BYHWD and BYJJF groups with different concentrations （0.1, 0.2, 0.5, 1.0, 2.0, 3.5 mg/mL）. MTT method was employed to determine the protective effects of BYHWD and BYJJF on model cells. Results Vivo studies showed that after 7 days of treatment with BYHWD and BYJJF, those determinated quotas were all significantly improved compared with MCAO model rats （P〈0.05）, and there were no differences between BYHWD and BYJJF （P〉0.05）. Vtiro studies showed that the protective effects of BYHWD and BYJJF took place 2 hours later, ano it was obvious in oxidative stress injury caused by H202, with statistical differences with model group （P〈0.05）. BYHWD and BYJJF could increase cell viability, and there was no difference between the groups with same concentration （P〉0.05）. Conclusion The research confirmed that BYJJF plays a significant role in improving the cerebral ischemia injury, which is the same performance as BYHWD, and BYJJF can save TCM resources under the precondition of TCM efficacy.
出处
《中国中医药信息杂志》
CAS
CSCD
2014年第9期46-49,共4页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家重点基础研究发展计划(2012CB723503)
湖南省中药粉体与创新药物省部共建国家重点实验室培育基地开放基金(ZYFT201307)
湖南省教育厅科研项目(13C672
13C683)
湖南省中医药管理局项目(2014135)
关键词
补阳还五汤
补阳还五汤精简方
脑缺血
局灶性脑缺血大鼠模型
氧化应激细胞模型
Buyang Huanwu Decoction
recipe composition of Buyang Huanwu Decoction
cerebral ischemic injury
MCAO rat model
oxidative stress cell model
