摘要
【目的】探讨苦瓜水提物(Momordica charantia fruit aqueous extract,MCFE)对拘束应激小鼠体内脂代谢及抗氧化水平的影响,为揭示苦瓜对脂代谢紊乱的改善作用提供理论依据。【方法】选用6周龄的雌性昆明小鼠,随机分为5组,包括正常对照组(NC)、拘束应激组(Mod)及-MCFE低(250 mg·kg-1)、中(500 mg·kg-1)和高(750 mg·kg-1)剂量组,每组12只,连续灌胃7 d后,置于50 mL带孔的塑料离心管中拘束20 h,尾静脉注射10%脂肪乳剂Interlipids,15 min后检测小鼠血脂消除能力,肠系膜脂肪组织脂肪酶活力,血清、肠系膜脂肪组织及肌肉组织的脂蛋白酯酶活性,同时测定血清的氧化状态及抗氧化水平。【结果】拘束应激模型组小鼠甘油三酯(Triglyceride,TG)的含量为正常组小鼠TG含量的1.27倍,而低、中、高剂量MCFE组的小鼠TG含量分别为正常组小鼠TG含量的0.63、0.57和0.55倍,表明MCFE各剂量组均能显著提高拘束应激小鼠甘油三酯的清除率(P<0.05),提高其脂肪代谢能力,并呈现剂量依赖性趋势;同时,拘束应激模型组小鼠的脂肪酶活性为正常组的0.52倍,而MCFE各剂量组的脂肪酶活性分别为正常组的0.77、0.98和1.06倍,明显高于拘束应激组(P<0.05),且中、高剂量组基本达到正常水平,表明MCFE各剂量组均能提高肠系膜脂肪组织的脂肪酶活性(P<0.05);此外,与拘束应激组相比,MCFE的低、中、高剂量组小鼠的血清脂蛋白酯酶(Lipoprotein Lipase,LPL)活性和肠系膜脂肪组织的LPL活性明显有所提高(P<0.05),亦表现剂量效应,其中MCFE各剂量组的血清LPL活性达到正常组水平,但MCFE各剂量组对肌肉组织的脂蛋白酯酶的活性影响不显著(P>0.05);另外,拘束应激组小鼠的脂质过氧化产物丙二醛(malondialdehyde,MDA)的含量是正常组的1.37倍(P<0.05),总抗氧化能力(ORAC)是正常组的0.65倍(P<0.05),而MCFE各剂量组小鼠体内的MDA含量明显低于拘束应激组(P<0.05),且MCFE的中、高剂量组基本达到正常水平,MCFE各剂量组ORAC明显高于拘束应激模型组,但尚未达到正常水平,表明MCFE能明显提高拘束应激小鼠体内总抗氧化水平(P<0.05)。【结论】苦瓜水提物能从氧化应激水平和脂质代谢两方面改善拘束应激小鼠脂代谢紊乱。
【Objective】The objective of this experiment is to study the effect of Momordica charantia fruit aqueous extract（MCFE） on lipids metabolism and oxidative situation in restraint mice and provide the theoretical basis for revealing the improving effect of M. charantia on lipids metabolism disorder.【Method】The 6 weeks old female mice were randomly divided into 5 groups including normal control group（NC）, stress control group（Mod）, three MCFE groups（250 mg·kg-1, 500 mg·kg-1 and 750 mg·kg-1）, which were administered samples once a day successively for 7 days. After intravenously injected 10% intralipids into the 20 h restrained mice, the content of serum lipid, lipase of mesenteric adipose tissue（MAT） and lipoprotein lipase（LPL） activity of different tissues were determined. The oxidative situation and the antioxidant capacity of the body were measured. 【Result】 The content of TG in Mod was 1.27 times of NC, but the content of low, medium and high MCFE groups were 0.63, 0.57, 0.55 times of NC. MCFE accelerated the elimination rate of TG（P0.05）in a dose-dependent manner, and improved the fatty acid metabolism. Lipase activity in Mod was 0.77 times of NC, and low, medium and high MCFE groups were 0.77, 0.98, 1.06 times of NC. Lipase activity of medium and high MCFE groups basically reached normal level. MCFE improved lipase activity of mesenteric adipose tissue（MAT）. Compared with the Mod, the LPL activity of serum and MAT in each MCFE group increased significantly with dose-dependent（P0.05）. But there was no significant effect on muscle LPL activity. The content of MDA and ORAC in Mod was 1.37 and 0.65 times of NC, respectively. The content of MDA of MCFE groups was lower than Mod significantly and the medium, high MCFE groups reached normal. The ORAC of MCFE groups was higher than Mod（P 0.05）. MCFE increased the antioxidant capacity of serum（ORAC）.【Conclusion】 MCFE improved the lipid metabolic dysfunction in restraint mice. The mechanism of M. charantia improves the lipid metabolic dysfunction in restraint mice may be the amelioration of oxidative situation in body and improving the LPL and LIPASE activities of related tissues.
出处
《中国农业科学》
CAS
CSCD
北大核心
2014年第16期3300-3307,共8页
Scientia Agricultura Sinica
基金
"十二五"国家科技支撑计划项目(012BAD33B10
12BAD31B03)
国家重点基础研究发展计划(2012CB722904)
国家级星火计划(2013GA780062)
省基金重点项目(10251064001000006)
关键词
苦瓜
水提取物
脂质代谢
氧化应激
小鼠
Momordica charantia
aqueous extract
lipid metabolism
oxidative stress
mice
