摘要
以胶体几丁质为唯一碳源,从大连渤海湾的底泥样品中分离到1株高产低温几丁质酶的海洋细菌,命名为DL-06。采用单因素优化方法确定该菌株产低温几丁质酶的最佳条件,结果表明:胶体几丁质5.0 g/L,胰蛋白胨5.0 g/L,发酵温度20℃,pH 7.0,2%接种量,装液量60%,转速130 r/min,陈海水1.0 L,发酵时间30 h,在该条件下酶活力达6.87 U/mL。
Using colloidal chitin as the sole carbon source, a marine bacteria with high productivity of cold-adapted chitinase was obtained from samples collected in Bohai bay in Dalian city of Liaoning Province, China,named as DL-06. The optimal fermentation conditions of cold-adapted chitinase-producing bacteria by single-factor experiments were determined as followed： colloidal chitin 5.0g/L, peptone 5.0 g/L, fermentation temperature 20℃, pH 7.0, inoculums dose 2%, shaking speed 130r/min, aged seawater 1.0 L and fermentation 30 h, respectively. Under these conditions, the maximum activity of cold-adapted chitinase was 6.87 U/ml.
出处
《大连大学学报》
2014年第3期82-87,共6页
Journal of Dalian University
关键词
海洋细菌
低温几丁质酶
优化
marine bacteria
cold-adapted chitinase
optimization
