摘要
目的探讨茶多酚(tea polyphenols,TP)对60Coγ射线诱导L5178Y细胞凋亡和细胞内活性氧水平(reactive oxygen species,ROS)的作用。方法 L5178Y细胞分为空白对照组、60Coγ射线辐照组、TP组及辐照加TP组共4组,TP组及辐照加TP组培养同时加入茶多酚终浓度分别为25、50、100μg/mL,60Coγ射线辐照组及辐射加TP组细胞给予8 Gy60Coγ射线一次性照射后,采用DCHF-DA探针测定细胞内活性氧(ROS)水平,Annexin V-FITC/PI双染和DNA琼脂糖凝胶电泳检测细胞凋亡。结果 8 Gy60Coγ射线照射后4 h,细胞活力明显降低,出现典型的细胞凋亡,琼脂糖凝胶电泳显示出现凋亡梯状条带,并伴随细胞内ROS水平明显升高,茶多酚能明显改善这些反应,TP处理组与辐照组相比有统计学差异(P<0.01)。结论茶多酚能显著降低60Coγ射线诱导的L5178Y细胞内活性氧产生,降低细胞凋亡率,对γ射线照射有一定的保护作用。
Objective To investigate the function of tea polyphenols （TP） on apoptosis and reactive oxygen species （ROS） level induced by 60Co γ rays in L5178Y cells. Methods L5178Y cells were divided into 4 groups： a control group, a γ-ray group, a TP group, and a TP＋γ-ray group. The TP group and TP＋γ ray group were treated with TP of 25, 50, and 100 μg/mL separately. After the γ-ray group and TP＋γ-ray group exposed to 8 Gy 60Co γ-ray radiation, Annexin V-FITC/PI staining and DNA agarose gel electrophoresis were used to detect the apoptosis of L5178Y cells. Intracellular ROS level was detected by DCFH-DA. Results Exposure to 8 Gy 60Coγ-ray radiation for 4 h, the L5178Y cells showed obvious reduction of cell viability, typical cell apoptosis, an increase of ROS level, and DNA ladder （DNA agarose gel electrophoresis）. TP could relieve the changes with statistic difference （P〈0.01）. Conclusion TP is able to suppress ROS production induced by 60Coγ-ray exposure and then reduce apoptotic rate of L5178Y cells. Therefore, TP is a protective agent for 60Coγ-exposure.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2014年第16期1699-1702,共4页
Journal of Third Military Medical University
