摘要
核仁小分子RNA(small nucleolar RNA,snoRNA)的主要功能是参与rRNA的修饰。最近研究表明,snoRNA可能具有广泛的生物学功能。大部分snoRNA比较保守,但也有一些snoRNA基因具有种属特异性。有意义的是,该研究发现,基因序列高度保守的snoRNA(SNORA50和SNORA71)呈现物种特异性的组织表达谱。SNORA50位于Cnot1基因内含子中,在脊椎动物中高度保守。表达谱分析发现,SNORA50在灵长类(人和恒河猴)和鸟类(鸡)的各种组织中广泛表达,但在正常发育的小鼠组织中检测不到。SNORA71位于一个非编码RNA基因snhg11的内含子中,在哺乳动物中比较保守。研究发现,SNORA71在灵长类各种组织广泛表达,而小鼠中主要在脑组织表达,与宿主基因snhg11表达谱一致。随小鼠脑发育过程的进展,SNORA71表达水平逐渐上调,提示其参与小鼠脑发育的调控。而在恒河猴从胚胎到成体的脑发育过程中,SNORA71表达没有显著的改变。这些结果表明,SNORA71对灵长类和啮齿类的神经发育可能存在不同的调控模式。基因序列保守的SNORA50和SNORA71呈现物种特异的表达模式,说明snoRNA在个体发育和系统进化中都发挥着重要的调控功能。
The small nucleolar RNA （snoRNA） functions mainly as modulators of ribosomal RNA （rRNA） Growing evidence suggested that snoRNA may have broader functions than previously appreciated. Although the majority of known snoRNAs are conserved during vertebrate evolution, some of them have been documented to be species-specific. Significantly, we herein found that two conserved intron-encoded snoRNAs, SNORA50 and SNORA 71, demonstrated species-specific tissue expression pattern, suggesting their functions in regulating indi- vidual organogenesis and phylogenesis. SNORA50, hosted by CCR4-NOT transcription complex, subunit 1 （Cnotl）, was ubiquitously expressed in various tissues of primate species （human and monkey） and bird （chicken）. However, there was no detectable signal of SNORA50 in all 8 examined mouse tissues. SNORA71, hosted by small nucleolar RNA host gene 11 （snhg11）, was pervasively transcribed in the human and monkey tissues. Interestingly, SNORA 71 was predominantly expressed in mouse brain as same as its host gene snhg11 did. SNORA 71 was upregulated during mouse brain development. However, no obvious upregulation was observed during monkey brain development from embryonic to adult stage, indicating that SNORA 71 might have different tUnctlons in regumung Hcutu^J development of primates and mouse species. Collectively, we have identified a neurogenically highly expressed SNORA 71 in mice, which provides important information to support snoRNA functions in regulating organogenesis during animal development. In addition, the species-specific transcriptional regulation of the conserved snoRNAs suggested their functional role in regulating individual development and phylogenesis.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2014年第8期1104-1109,共6页
Chinese Journal of Cell Biology
基金
国家重点基础研究发展计划(973计划)(批准号:2007CB946903)资助的课题~~
