摘要
目的:运用快速成型技术(RP)构建聚己内酯(PCL)支架,检测其生物相容性,探讨成为组织工程骨支架的潜力。方法:以聚己内酯为原料利用熔融沉积成型法(FDM)制备无孔隙及孔径300μm、500μm 3种PCL支架,比重瓶法测孔隙率。利用MTT法检测支架材料对细胞增殖的影响。倒置荧光显微镜、扫描电子显微镜观察聚己内酯支架上的细胞粘附情况及细胞形态。结果:肉眼观察可见300μm及500μm孔径支架材料孔隙大小均匀,排列规整,层次分明,两种孔径支架都具有良好的孔隙连通率。MTT检测结果显示1d、2d、3d各时间点均无明显细胞毒性,细胞毒性为1级。荧光倒置显微镜下观察发现细胞粘附数量由无孔隙组、500μm孔径组、300μm孔径组依次增加。扫描电子显微镜下观察细胞紧密粘附于支架。结论:用快速成型技术制备的聚己内酯支架具有较高的孔隙率,孔隙之间连通率良好,生物相容性良好,细胞粘附良好,有望成为骨组织工程支架。
Objective： To test the biocompatibility of PCL scaffold and explore the potential as bone tissue engi- neering scaffolds. Methods： Two PCL scaffolds were prepared in two sizes （300μm and 500μm） by fused deposition modeling （FDM）. The scaffolds appearance under the inverted microscope was observed and the apertures was measured. The influences on cell viability by MTT. The cell adhesion on scaffolds was observed by fluorescence microscope and electron microscope. Results： The holes of PCL scaffolds were equirotal, marshal and well-bedded by the inverted fluorescence microscope. The timbering materials＇ apertures were 300μm and 500μm. Both scaffolds had good connexity. All scaffolds had no significant cytotoxicity in 1, 2 and 3 days according to the MTT results. The toxicity grade was 1. We could find that the number of cell adhesion by no pore group, 500 microns aperture group and 300 microns diameter increase in turn. It could be observed that the cells were tightly adhered to the scaffolds. Conclusion： The PCL scaffold that prepared by RP has fine porosity, excellent biocompatibility and well cell adhesion.
出处
《口腔医学研究》
CAS
CSCD
2014年第8期713-716,共4页
Journal of Oral Science Research
