摘要
目的用RP-HPLC分离广金钱草中的夏佛塔苷和异夏佛塔苷,并对其进行定量测定。方法色谱柱为依利特C18（4.6 mm×250 mm,5μm）;流动相为乙腈-0.025 mol/L磷酸溶液（三乙胺调pH至3.02）（15∶85）;柱温30℃;体积流量0.9 mL/min;检测波长270 nm。结果广金钱草中夏佛塔苷的线性方程为Y=2×106X-153 980,进样量在0.158~0.898μg范围内线性关系良好（r=0.999 7）,回收率为100.0%,RSD为0.7%;异夏佛塔苷的线性方程为Y=3×106X-86 359,进样量在0.076~0.436μg范围内线性关系良好（r=0.999 6）,回收率为99.9%,RSD为0.6%。结论增加异夏佛塔苷的色谱检测有利广金钱草及其制剂的质量控制。
AIM To establish a RP-HPLC method for separating and determining the contents of schaftoside and isoschaftoside from Desmodium styracifoolium （ Osb. ） merr. METHODS The chromatographic analysis was performed on C ls column （4. 6 mm × 250 mm, 5 μm）, the mobile phase was acetonitrile -0. 025 mol/L phosphor- ic acid solution （ adjusted triethylamine to pH = 3.02） （ 15 ： 85 ） and the flow rate was 0.9 mL/min. Column tem- perature was maintained at 30 ℃ Detection wavelength was set at 270 nm. RESULTS The linear equation of schaftoside was Y = 2 × 106X - 153 980 with a good linear relationship in the range of 0. 158 - 0. 898 μg （ r = 0. 999 7 ） , the recovery rate was 100. 0% , and RSD was 0. 7% . The linear equation of isoschaftoside was Y = 3 × 106X-86 359 with a good linear relationship in the range of 0. 076 -0. 436 μg （r =0. 999 6）, the recovery rate was 99.9% , and RSD was 0. 6% . CONCLUSION Adding chromatographic inspection of isoschaftoside is fa- vorable to the quality control of Desmodium styracifolium （ Obs. ） merr and its preparations.
出处
《中成药》
CAS
CSCD
北大核心
2014年第9期1909-1912,共4页
Chinese Traditional Patent Medicine
