摘要
为了研究牦牛ANT4在脑组织中的表达情况,试验通过RT-PCR技术克隆牦牛SLC25A31基因的cDNA序列,并利用DNAMAN、MAGA4、SWISS-MODEL、ExPASy等生物信息学软件系统对其进行分析。结果表明:扩增出的牦牛SLC25A31基因的编码区长972 bp,编码323个氨基酸;与普通牛、鼠和人相应基因核苷酸序列进行比对,序列一致性分别为99.28%、84.88%和89.81%;其编码蛋白分子质量为35.695 ku,含多个修饰位点。说明SLC25A31基因的CDS序列及其表达蛋白的结构在高原牦牛与普通牛间并无明显差异,保守性极强;并且通常在睾丸中表达的ANT4在高原牦牛脑组织中也能表达。
To study the expression of ANT4 in brain tissues of yak （Bos grunniens）, the cDNA sequence of yak SLC25A31 was cloned by RT - PCR, and was also analyzed by bioinformatics software systems including DNAMAN, MAGA4, SWISS - MODEL, ExPASy, etc, respectively. The results showed that the length of coding area for the amplified SLC25A31 gene of yak was 972 bp, encoding for a protein of 323 amino acids. Compared with nucleotide sequences of Bos taurus, Mus musculns, Homo sapiens, the sequence identities were 99.28%, 84.88%, 89.81%, respectively. The molecular weight of the encoded protein was 35. 695 ku, containing multiple modification sites. The results indicate that there are no obvious differences in the CDS sequence of the SLC25A31 gene and the structure of its expression protein between the plateau yak and ordinary cattle, and they are highly conservative; ANT4, which is usually expressed in the testis, can also be expressed in the brain tissue of plateau yak.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2014年第9期29-32,227,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(31260588)
青海省自然科学基金项目(2011-Z-901)
