摘要
红松（ Pinus koraiensis）是我国东北地区的优势树种，经济价值极高。松多酚是从红松组织中分离出来的一类次生代谢产物，具有很强的生理活性，有助于预防癌症和心脑血管疾病的发生（Morton et al．，2000； Tassoni et al．，2012）。松多酚作为一种天然、无毒、高效的抗氧化剂，可以广泛应用于食品、保健品和医药领域，因此如何采取有效的措施提高松多酚的含量具有重要意义。稀土是一类具有生理活性的元素，可以对植物产生多种生理效应、提高种子萌发率、促进根系发育、提高生物量和增强抗逆性等，在农业上得到广泛应用（ Arora et al．，2002； Hu et al．，2002；2004）。随着研究的不断深入，稀土元素（镧、铕、铈、镱等）的应用已经拓展到植物细胞和组织培养领域（Chen et al．，2004）。
Pinus koraiensis seedlings were used as experimental material to study the effects of rare earth elements La and Eu on the growth,polyphenols contents and PAL,C4H activities of P. koraiensis seedlings. The results showed that rare earth elements La and Eu could significantly promote the growth and increase the polyphenols contents of P. koraiensis seedlings. The optimum concentrations of La3 ＋ and Eu3 ＋ were 400 μmol·L -1 and 100 μmol·L -1 ,respectively. With the concentrations,the polyphenols contents and were 1. 55 and 1. 39 times higher,and the proanthocyanidins contents were 1. 58 and 1. 40 times higher than the control group,respectively. The PAL and C4H activities significantly increased and peaked in two days after the induction with 100 μmol · L -1 La3 ＋ and 400 μmol·L -1 Eu3 ＋. There were significant correlations between the enzymes activities and the synthesis of polyphenols. The 100 μmol · L -1 La3 ＋ showed more significantly promoting effects than the 400 μmol·L -1 Eu3 ＋ on both P. koraiensis seedlings growth and polyphenols synthesis. Rare earth elements La3 ＋ and Eu3 ＋ may have acted as inductor of P. koraiensis secondary metabolism,elicitor-induce defensive responses in P. koraiensis,activate the phenylpropanoid pathway,change the activity of related enzymes,and thus enhance polyphenols synthesis.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2014年第8期168-173,共6页
Scientia Silvae Sinicae
基金
国家自然科学基金项目(31170510)
