摘要
目的：探讨模拟失重30 d后再超重对猴舌黏膜中趋化因子CCL20及其受体CCR6表达的影响。方法23只雄性猕猴采用随机数字表法分为对照组（A组，n＝3）、失重组（B组，n＝3）、超重组（ C组，n＝3）、失重30 d后再超重组（ D组，n＝14）；D组根据载荷量又分为4个亚组：＋11Gx/270s组（D1，n＝3）、＋13Gx/230s组（D2，n＝4）、＋15Gx/200s组（D3，n＝4）和＋13Gx/230s恢复9 d后组（ D4，n＝3）。采用组织学方法观察舌黏膜的形态学变化，通过免疫组织化学、实时定量PCR方法检测舌黏膜中CCL20、CCR6的表达情况。结果舌黏膜在各实验组中组织结构均未见明显损伤，但实验组中的淋巴细胞及中性粒细胞浸润均较对照组多。 C组、D2组及D3组CCL20的免疫组化评分分值分别为1.30±0.11、1.68±0.62和2.26±1.00，均显著高于A组的0.47±0.12，差异均有统计学意义（均P＜0.05），CCR6的免疫组化评分分值分别为4.40±1.48、6.67±2.04和7.02±2.11，也显著高于A组的1.33±0.66，差异均有统计学意义（均P＜0.05）。在mRNA水平上，各组的CCL20和CCR6表达水平变化趋势与其蛋白水平变化一致。结论模拟失重30 d后再超重环境中，猴舌黏膜组织结构未见明显改变，但舌黏膜中趋化因子CCL20及其受体CCR6表达明显增强。
Objective To study the influence of hypergravity exposure after 30-days simulated weightlessness on the expression of chemokine CCL 20 and its receptor CCR6 in lingual mucosa of rhesus macaque.Methods Twenty-three male rhesus monkeys were divided according to the random number method into one control group （A, n=3） and three experimental groups, including the weightlessness group （ B, n =3 ） , the hypergravity group （ C, n =3 ） and hypergravity exposure after 30-days simulated weightlessness group （D, n=14）, which was further divided into four subgroups according to the values of overload as： ＋11 Gx/270s group （D1, n=3）, ＋13 Gx/230s group （D2, n=4） and ＋15 Gx/200s group （D3, n=4） and ＋13 Gx/230s with 9-days recovery group （D4, n=3）.Histopathological changes of lingual mucosa were observed by hematoxylin-eosin staining and the expressions of CCL 20 and CCR6 were detected by immunohistochemistry （ IHC） and quantitative real-time PCR （ Q-PCR）.Results Histological observation showed that no significant histopathological changes were found in the lingual mucosa in all experimental groups of the animals ,however, there were more infiltrated lymphocyte and neutrophils in the experimental groups.The immunohistochemical scores of CCL 20 in group C, group D2 and group D3 were 1.30±0.11, 1.68±0.62 and 2.26±1.00, respectively, significantly higher than that in A group （0.47± 0.12,P〈0.05）.The immunohistochemical scores of CCR 6 expression in those three groups were 4.40 ± 1.48, 6.67 ±2.04 and 7.02 ±2.11, respectively, also remarkably higher than that in A group （1.33 ±0.66,P〈0.05）.As far as the mRNA expression was concerned , the expressions of CCL20 and CCR6 had similar change trend with their protein expressions.Conclusion Hypergravity exposure after 30-days simulated weightlessness will not lead to significant pathological changes in lingual mucosa , but can induce the expression of chemokine CCL 20 and its receptor CCR6.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2014年第32期2525-2530,共6页
National Medical Journal of China
基金
国家自然科学基金（30672319）
全军医药卫生科研基金（06MA004）
