摘要
目的：建立吴紫糊中左旋紫草素的测定方法。方法：高效液相色谱法,色谱条件色谱柱为安捷伦C18柱（4.6mm×250mm,5μm）,流动相为甲醇-0.1%磷酸盐缓冲液（80∶20）,检测波长516nm,柱温25℃,流速10mL/min。结果：进样量在8.2∽41.0μg/mL范围内,线性关系良好（r=0.999 9）平均回收率为101.30%（RSD为1.55%,n=9）。结论：高效液相色谱法测定吴紫糊中左旋紫草素含量结果准确、重现性好,适用于吴紫糊的质量控制。
Objective： To establish the determination method for shikonin in WuZi cataplasm. Methods： High performance liquid chromatography （HPLC）, chromatographic condition, chromatographic column： agilent C18 column （4.6 min×250 mm, 5 μm）, mobile phase： methanol-0.1% phosphate buffer solution （80：20）, detection wavelength： 516nm, column temperature：25℃, flow rate was 10mL/min. Results： Sample size was in the range between 8.2 and 41.0 μg/mL, linear relationship was better （r=0.999 9）, average recovery rate was 101.30% （RSD was 1.55%, n=9）. Conclusion： HPLC determining the contents of shikonin in IguZi cataplasm is accurate and repro- ducible, which could be applied to quality control ofWuZi cataolasm.
出处
《西部中医药》
2014年第8期14-16,共3页
Western Journal of Traditional Chinese Medicine
基金
白银市2010年第二批科技计划项目(编号GK20103-2-059A)
关键词
吴紫糊
高效液相色谱法
左旋紫草素
含量测定
WuZi cataplasm
high performance liquid chromatography
shikonin
content determination
