摘要
目的探讨急性暴露在高原缺氧环境(海拔4300 m)对Wistar大鼠体质量、血清中TNF-α和一氧化氮(NO)含量,脑和肺生理病理的影响及氨茶碱干预对缺氧大鼠的多重保护作用。方法将21只成年健康Wistar大鼠随机分为高原空白组(0.9%氯化钠注射液,每日3次,每次0.5 ml)、高原给药组(氨茶碱,每日3次,剂量17.86 mg·kg-1)、高原对照组(乙酰唑胺,每日2次,剂量22.33 mg·kg-1),药物均以0.9%氯化钠注射液溶解配制。大鼠进入高原24 h后开始给药,连续灌胃给药5 d后,解剖大鼠,完整摘取脑、肺组织进行病理观察,期间于实验第1、5天称体质量,于实验第1、3、5天取血样,检测血清中的TNF-α、NO含量。结果大鼠急进4300 m高原后,在缺氧适应5 d过程中,体质量变化情况分别为:空白组下降2.4%,给药组上升3.2%,对照组下降6.5%;第5天血清促炎因子TNF-α浓度变化情况为:空白组(184.85±24.09)ng·L-1,给药组(208.79±22.23)ng·L-1,对照组(213.41±15.26)ng·L-1;第5天血清NO浓度为:空白组(19.81±0.87)μmol·L-1,给药组(22.24±1.49)μmol·L-1,对照组(21.91±1.78)μmol·L-1。病理切片显示,氨茶碱组大鼠脑、肺组织损伤亦较高原空白组大鼠减轻。结论氨茶碱通过促进营养的吸收、炎症应激的快速反应、改善呼吸功能及调节NO舒血管作用,达到对急进高原大鼠的多重保护作用。
Objective To investigate the effect of amimophylline on acute exposure to high altitude environments（ 4300 m） on the body weight,serum TNF-α and NO contents,brain,lung of Wistar rats. Methods 21 healthy adult Wistar rats were randomly divided into high altitude group（ Group One,0. 9% sodium chloride injection0. 5 ml,three times a day）,high altitude treatment group（ Group Two,aminophylline 17. 86 mg·kg- 1,3 times a day）,and high altitude control group（ Group Three,acetazolamide 22. 33 mg·kg- 1,2 times a day）. Drugs were given at 24 h after entering the plateau. After being oral administered for 5 consecutive days,the rats were dissected and brain and lung tissues were obtained for pathological observation. The TNF-α and NO contents were detected from the blood samples at day 1,3 and 5. Results In the course of five-day adaptation to hypoxia,body weight decreased by 2. 4% in Group One,increased by 3. 2% in Group Two,and decreased by 6. 5% in Group Three. The concentration of serum proinflammatory cytokine TNF-α at day 5 was（ 184. 85 ± 24. 09） ng·L- 1in Group One,（ 208.79 ±22.23） ng·L- 1in Group Two,and（ 213. 41 ± 15. 26） ng·L- 1in Group Three. Conclusion Aminophylline can produce multiple protective effect on acute exposure to high altitude environments by promoting absorption of nutrients,response to inflammatory stress,respiratory functions and vascular effects of NO.
出处
《解放军药学学报》
CAS
2014年第4期277-280,共4页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
国家科技部重大资助项目
No.2008ZXJ09014-010
全军医药科研"十二五"重点项目
No.BWS12J012
全军医药科研"十二五"面上项目
No.CWS11C231
