摘要
文章以8日龄矮小型黄羽肉雏鸡为材料,以28℃±1℃处理3 h为对照组,40℃±1℃热应激3 h为实验组,采集大脑、肝脏、腿肌3种组织,利用全基因组表达谱芯片检测实验组和对照组各组织的差异表达基因,并结合生物信息学方法分析其功能。研究结果表明:在热应激处理组与对照组中,大脑组织中共有183个显著变化的差异表达探针(变化倍数≥2或≤0.5,q≤0.05),129个有基因注释,39个生物学过程发生了改变,18个生物学通路发生显著改变;肝脏组织中有558个显著改变的探针(变化倍数≥2或≤0.5,q≤0.05),包含339个基因,涉及37个生物学过程,58个生物学通路发生改变;腿肌组织中显著改变的探针有301个(变化倍数≥2或≤0.5,q≤0.05),209个有基因注释,参与了31个生物学过程,51个生物学通路发生改变。3种组织中,获得差异表达探针935个,不同组织具有特异性的表达模式;多种热休克蛋白家族基因被诱导上调表达,抗凋亡过程、钙离子信号通路、MAPK信号通路和多种代谢途径发生改变。文章对鸡的热应激分子机制进行了初步探索,为筛选耐热性相关基因提供了理论依据。
In the present study, tissue samples were collected from the cerebrum, liver, and leg muscle of 8-day-old dwarf chicks that were exposed to a 3 h treatment of 28℃± 1 ℃ （control group） or 40℃± 1 ℃ （treatment group）. Differen- tially expressed （DE） genes in these samples were detected using whole-genome microarray chips, and their functions werepredicted with bioinformatics methods. The results show that after the heat treatment, the expression of 183 DE genes had significantly changed in cerebrum tissue samples （fold change ≥ 2 or ≤ 0.5, q values ≤ 0.05）; of these, 129 genes were an- notated, and 39 biological processes and 18 distinct biological pathways the expression of 558 DE genes had significant- ly changed （fold change ≥ 2 or ≤ 0.5, q values ≤ 0.05）; of these, 339 were found to be significantly upregulated. In liver tissue samples, genes were annotated, and 37 biological processes and 58 biological pathways were found to be significantly upregulated. In leg muscle tissue samples, the expression of 301 DE genes had significantly changed （fold change ≥ 2 or ≤ 0.5, q values ≤ 0.05）; of these, 209 genes were annotated, and 31 biological processes and 51 biological pathways were found to be significantly upregulated. In total, 935 DE genes exhibiting a change in expression were identified in the samples of the 3 tissues analyzed in this study; these different tissues exhibited distinct gene expression patterns. The expression of various heat shock protein （HSP） genes was upregulated, and anti-apoptosis, calcium signaling, MAPK signaling, and other metabolic pathways were altered in these tissues. Thus, this study provides fundamental evidence for further identifying heat toler- ance-related genes.
出处
《遗传》
CAS
CSCD
北大核心
2014年第8期800-808,共9页
Hereditas（Beijing)
基金
国家自然科学基金项目(编号:30972093)
国家科技支撑计划项目(编号:2014BAD08B08)资助
关键词
鸡
热应激
表达谱
基因芯片
chicken
heat stress
gene expression profiling
gene chip
