摘要
比较评价当归-红花(GH)不同配比对急性血瘀大鼠血液流变学和凝血功能的影响,优选当归-红花配伍活血作用的最佳配比。采用皮下注射盐酸肾上腺素和冰水游泳共同复制急性血瘀大鼠模型,通过测定全血黏度(WBV)、血浆黏度(PV)、全血高切相对指数(HSWBRI)、全血低切相对指数(LSWBRI)、红细胞聚集指数(EAI),观察当归-红花配伍对血瘀大鼠血液流变学的影响;通过测定凝血酶原时间(PT)、凝血酶时间(TT)、活化部分凝血活酶时间(APTT)、血浆纤维蛋白原(FIB)含量及血小板最大聚集率(ADP),观察当归-红花配伍对血瘀大鼠凝血功能及血小板聚集的影响。然后采用主成分分析法和多指标综合指数法综合评价当归-红花配伍的总活血化瘀效应。结果显示,与正常组比较,模型组各血液流变学指标和凝血功能指标均有显著性差异;与模型组比较,当归-红花不同配比(1∶0、4∶1、2∶1、3∶2、1∶1、2∶3、1∶2、1∶4和0∶1)组均能较好改善急性血瘀大鼠的血液流变学各指标,对凝血功能多个指标也有较好的调节作用。主成分分析法和多指标综合指数法综合评价得出当归-红花配比1∶1和3∶2的活血化瘀作用最好,其中1∶1的活血化瘀作用更优于3∶2。以上结果说明,当归-红花配伍能明显改善血瘀大鼠的血液流变学及凝血功能异常,且当归-红花活血化瘀作用的最佳配比与临床中医方剂中使用当归-红花1∶1频次最高的结论相一致,为临床更有效应用当归-红花配伍提供了科学依据。
The combination of Danggui and Honghua （GH） is a popular herb pair commonly used in clinic for the treatment of blood stasis syndrome in China. To evaluate the activating blood circulation and dissipating blood stasis effects of the combination of different proportions of Danggui and Honghua on acute blood stasis rats, and optimize the proportion of GH to have the best activating blood circulation and dissipating blood stasis effect. Acute blood stasis rat model was induced by subcutaneous injection of adrenaline and ice water bath.The blood stasis rats were administrated intragastrically with GH （1 ： 0, 4 ： 1, 2 ： 1, 3 ： 2, 1 ： 1, 2 ： 3, 1 ： 2, 1 ： 4 and 0 ： 1） extracts. The whole blood viscosity （WBV）, plasma viscosity （PV）, and high shear whole blood relative index （HSWBRI）, low shear whole blood relative index （LSWBRI）, and erythrocyte aggregation index （EAI） were tested to observe the effects of GH on hemorheology of blood stasis rats. And the maximum aggregation induced by adenosine diphosphate （ADP） was tested to observe the effect of GH on platelet aggregation index of blood stasis rats. In addition, the prothrombin time （PT）, thrombin time （TT）, activated partial thromboplastin time （APTT） and plasma fibrinogen （FIB） were tested to observe the effects of GH on blood coagulation function of blood stasis rats. Then principal component analysis and multi-attribute comprehensive index methods were both used to comprehensively evaluate the total activating blood circulation and dissipating blood stasis effects of GH. The results showed that the hemorheological indexes and coagulation parameters of model group both had significant differences with normal group. Compared with model group, GH （1 ： 0, 4 ： 1, 2 ： 1, 3 ： 2, 1 ： 1, 2 ： 3, 1 ： 2, 1 ： 4 and 0 ： 1） could improve all the blood hemorheology indexes and regulate part indexes of blood coagulation function and platelet aggregation in acute blood stasis rats. Based on principal component analysis and multi-attribute comprehensive index methods, GH 1 ： 1 and GH 3 ： 2 both had the best effect of blood circulation and dissipating blood stasis, and the effect of GH 1 ： 1 was slightly better than GH 3 ： 2. These results suggest that GH could obviously ameliorate the abnormality of hemorheology and blood coagulation function in acute blood stasis rats. The optimized proportion of GH was consistent with regulations of medicine usage that GH 1 ： 1 had the highest frequency used in traditional Chinese formulae. It could provide scientific basis for more effective application of the compatibility between Danggui and Honghua in modern clinic medicine.
出处
《药学学报》
CAS
CSCD
北大核心
2014年第9期1304-1309,共6页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(81274058)
江苏高校优势学科中药学学科建设工程资助项目(PAPD)
关键词
当归
红花
配伍
活血化瘀
主成分分析
多指标综合指数法
Danggui
Honghua
compatibility
blood circulation and dissipating blood stasis
principal component analysis
multi-attribute comprehensive index method
