摘要
背景:不同来源的干细胞具有不同的分子特征及生长特性,对疾病治疗的机制及效果可能会有所差异。目的:比较两种来源间充质干细胞的生物学特性。方法:分离培养人脐带源、胚胎肝来源的间充质干细胞,传至第5代时分别进行细胞形态观察,细胞群体倍增时间计算,细胞表面标志鉴定,诱导分化能力检测。另外将脐带源间充质干细胞继续培养至第10,15代,绘制生长曲线并计算细胞群体倍增时间。结果与结论:两种来源的间充质干细胞在对数生长期均为梭形,旋涡状生长,均具有成骨、成脂、成软骨分化的能力。两种间充质干细胞的生长曲线均为S形。第5代胎肝来源间充质干细胞的倍增时间为(34.37±0.31)h,脐带源间充质干细胞的倍增时间为(35.63±0.38)h,差异无显著性意义(P>0.05)。第10代和第15代脐带源间充质干细胞的倍增时间分别为(52.6±0.53)h和(53.27±0.92)h,与第5代比较差异有显著性意义(P<0.05)。结果可见两种来源间充质干细胞生物学特性基本相同,肉眼观察胎肝来源间充质干细胞比脐带源间充质干细胞形态稍小,增殖较快,但统计学上差异无显著性意义。
BACKGROUND： Different sources of stem cells have different molecular characteristics and growth characteristics; therefore, there are some differences in therapeutic mechanisms and effects. OBJECTIVE： To compare mesenchymal stem cells growth characteristics form two sources. METHODS： Mesenchymal stem cells from the umbilical cord and the embryonic liver were isolated and cultured. Passage 5 cells were used to observe the cell morphology, calculate the doubling time of cell population-doubling time, identify surface markers and determine the differentiation capacity. Mesenchymal stem cells from the umbilical cord were subcultured to passages 10 and 15, and cell curves were drawn and population doubling time was calculated. RESULTS AND CONCLUSION： Mesenchymal stem cells from these two sources in logarithmic phase were fusiform and grew spirally with osteogenic, adipogenic, and chondrogenic capacities. The growth curves of cells were both S-shaped. At passage 5, the doubling time was（34.37±0.31） hours for embryonic liver-derived mesenchymal stem cells and（35.63±0.38） hours for umbilical cord-derived mesenchymal stem cells, and there was no significant difference（P 〉 0.05）. However, the population doubling time of passages 10 and 15 umbilical cord-derived mesenchymal stem cells was（52.6±0.53） and（53.27±0.92） hours, respectively, which was significantly difference from that of passage 5 cells（P 〈 0.05）. The cell morphology and growth curve from two sources are basically the same. Embryonic liver-derived stem cells are smaller and proliferate faster than umbilical cord-derived mesenchymal stem cells, but no statistical difference is found between the two types.
出处
《中国组织工程研究》
CAS
CSCD
2014年第32期5166-5172,共7页
Chinese Journal of Tissue Engineering Research
