高效液相色谱法同时测定栀子金花丸中9个成分的含量被引量：10

Simultaneous determination of nine compounds in Zhizi Jinhua pills by HPLC wavelength switching method

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摘要目的：建立高效液相色谱法同时测定栀子金花丸中9个成分的方法。方法：采用DIKMA Platisil ODS色谱柱（250 mm×4.6 mm,5μm）,柱温35℃,流动相为甲醇-0.1%磷酸水溶液,梯度洗脱（0-5 min,40%A;5-20 min,40%A→60%A;20-26 min,60%A→60%A;26-35 min,60%A→90%A;35-52 min,90%A;52-54 min,90%A→40%A）,流速为1 mL·min-1,变换波长检测（327 nm,绿原酸、盐酸小檗碱;254 nm,栀子苷、黄芩苷、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚）。结果：绿原酸、栀子苷、盐酸小檗碱、黄芩苷、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的线性范围分别为0.02-0.4μg（r=0.9998）、0.049-0.98μg（r=1.000）、0.05-1μg（r=0.9999）、0.1-2μg（r=1.000）、0.0025-0.05μg（r=0.9996）、0.001-0.02μg（r=0.9997）、0.0014-0.028μg（r=0.9998）、0.004-0.08μg（r=0.9998）、0.00033-0.0066μg（r=0.9998）,平均回收率在98.8%-100.4%之间。结论：该法经方法学验证,是可用于栀子金花丸质量控制的一个有效的方法。 Objective：To establish a method for simultaneous determination of chlorogenic acid,geniposide,berberine,baicalin,aloeemodin,rhein,emodin,chrysophanol and physcion in Zhizi Jinhua pills. Methods：The separation was performed on a DIKMA Platisil ODS column（ 250 mm × 4. 6 mm,5 μm） with gradient elution（ 05 min,40% A; 520 min,40% A→60% A; 2026 min,60% A→60% A; 2635 min,60% A→90% A; 3552 min,90% A; 5254 min,90% A→40% A） using methanol and 0. 1% phosphoric acid aqueous solution as the mobile phase. The flow rate was 1 mL·min1and the detection wavelengths were 327 nm for chlorogenic acid and berberine,254 nm for geniposide,baicalin,aloeemodin,rhein,emodin,chrysophanol and physcion,respectively.Results：Linear ranges of chlorogenic acid,geniposide,berberine,baicalin,aloeemodin,rhein,emodin,chrysophanol and physcion concentrations were 0. 020. 4 μg（ r = 0. 9998）,0. 0490. 98 μg（ r = 1. 000）,0. 05-1 μg（ r = 0. 9999）,0. 12 μg（ r = 1. 000）,0. 00250. 05 μg（ r = 0. 9996）,0. 0010. 02 μg（ r = 0. 9997）,0. 00140. 028 μg（ r = 0. 9998）,0. 0040. 08 μg（ r = 0. 9998）,0. 000330. 0066 μg（ r = 0. 9998）,respectively. Their average recoveries fell between 98. 8% and 100. 4%. Conclusion：The methodology validation shows that this HPLC method established can be used to determine the contents of chlorogenic acid,geniposide,berberine,baicalin,aloeemodin,rhein,emodin,chrysophanol and physcion in Zhizi Jinhua pills. The method is an effective method for the quality control of Zhizi Jinhua pills.

作者郝乘仪郭淑英朱鹤云冯波

机构地区吉林医药学院药学院药物分析教研室

出处《药物分析杂志》 CAS CSCD 北大核心 2014年第9期1571-1575,共5页 Chinese Journal of Pharmaceutical Analysis

关键词栀子金花丸绿原酸栀子苷盐酸小檗碱黄芩苷芦荟大黄素大黄酸大黄素大黄酚大黄素甲醚高效液相色谱法中成药多组分分析 Zhizi Jinhua pills chlorogenic acid geniposide berberine baicalin aloe-emodin rhein emodin chrysophanol physcion HPLC multicomponent analysis of Chinese patent medicine