摘要
通过尾静脉注射四氧嘧啶诱导糖尿病小鼠模型,测定血糖、肝糖元以及血清中TG、TCH、MDA和SOD的变化,观察黑莓籽石油醚、乙酸乙酯和正丁醇部位以及黑莓籽油对糖尿病小鼠血糖、脂质代谢和氧化应激的影响。结果表明,石油醚部位低剂量组能显著的升高肝糖元的含量(p<0.05),降低TG(p<0.01)、TCH(p<0.05)和MDA的含量(p<0.05);乙酸乙酯部位高剂量组能显著的升高肝糖元的含量(p<0.05),降低TG(p<0.05)和TCH含量(p<0.05);乙酸乙酯部位中剂量组能显著性的升高肝糖元的含量(p<0.01),降低TG(p<0.05)和TCH含量(p<0.05),升高SOD的水平(p<0.05);黑莓籽油高剂量组能显著性的降低TG(p<0.05)和TCH含量(p<0.05);黑莓籽油低剂量组能显著性的升高肝糖元的含量(p<0.05),降低TCH含量(p<0.05)。可见,黑莓籽石油醚和乙酸乙酯部位及黑莓籽油对小鼠糖尿病降血糖作用不显著,可以促进肝糖元合成,抑制肝糖元分解,纠正脂质代谢紊乱和增强机体抗氧化防御体系。
The diabetic mouse models were established by intravenous injection of alloxan, the levels of blood sugar,hepatic glycogen and TG,TCH,MDA and SOD in serum were detected to evaluate hypoglycemic activity, lipid metabolism and oxidative stress of blackberry seed. The results showed that low dose group of petroleum ether extract could significantly decrease the content of liver glycogen（p 〈 0.05）, and increase the contents of TG （p 〈 0.01 ）, TCH （ p 〈 0.05 ） and MDA （ p 〈 0.05 ）. High dose group of ethyl acetate extract could significantly decrease the content of liver glycogen （ p 〈 0.05 ）, and decrease the contents of TG （ p 〈 0.05） and TCH （ p 〈 0.05 ）. Medium dose group of ethyl acetate extract could significantly increase the content of liver glycogen （ p 〈 0.0] ）, and decrease the contents of TG （ p 〈 0.05） and TCH （ p 〈 0.05 ）, and increase the level of SOD （ p 〈 0.05）. High dose group of blackberry seed oil could significantly decrease the contents of TG （ p 〈 0.05） and TCH （ p 〈 0.05）. Low dose group of blackberry seed oil could significantly increase the content of liver glycogen （p 〈 0.05）, and decrease the content of TCH （p 〈 0.05）. It provided evidence that the petroleum ether extract, ethyl acetate extract and oil of blackberry seed had no significant hypoglycemic effect,and could promoted hepatic glycogen synthesis and inhibited excessive degradation, rectified the derangement in lipide metabolism and strengthened antioxidant defense system.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第19期351-354,358,共5页
Science and Technology of Food Industry
基金
河南省高等学校青年骨干教师资助计划(2013GGJS-220)
郑州市科技局科技攻关计划(20120684
20140790)
河南省科技厅重点科技攻关(142102310147)
关键词
黑莓籽
糖尿病
降血糖作用
blackberry seed
diabetes
hvooalvcemic effect
