摘要
以橡胶树品种RRII105花药为外植体,研究了不同浓度2,4-D、KT、NAA及TDZ组合对橡胶树花药愈伤组织诱导的影响。结果表明:最适合诱导橡胶树花药愈伤组织的培养基配方为MS+0.8mg/L 2,4-D+0.8mg/L KT+1.2mg/L NAA+0.0mg/L TDZ+60g/L蔗糖+5g/L琼脂,诱导率达94.00%,出胚率为14.25%。
Taking anther of Hevea brasiliensis clone RRII105 as explants,the influence of different concentrations of plant growth regulators（2,4-D,KT,NAA,TDZ）on callus induction of anther were studied.The results showed that the best medium was MS＋0.8mg/L 2,4-D＋0.8mg/L KT＋1.2mg/L NAA＋0.0mg/L TDZ＋60g/L sucrose＋5g/L agar.The inductive rate of callus was 94.00%,the inductive rate of embryoid was 14.25%.
出处
《北方园艺》
CAS
北大核心
2014年第18期109-111,共3页
Northern Horticulture
基金
国家科技支撑计划资助项目(2011BAD30B01)
云南省产业重大关键技术开发专项计划资助项目(云发改[2008]1657号)
云南省应用基础研究自筹经费资助项目(2013FZ169)
2014云南省热带作物科技创新体系建设资金资助项目(RF2014)
云南省科研院所技术开发研究专项资助项目(2013DC013)
关键词
正交实验
橡胶树
花药
愈伤组织
orthogonal experiment
Hevea brasiliensis
anther
callus
