摘要
目的 观察糖尿病患者及糖尿病合并冠心病患者尿8-羟基脱氧鸟苷（8-OHdG）水平.方法 来自天津医科大学总医院住院患者及健康查体中心体检人群150名,分为糖尿病组（50例,其中男性25例,女性25例）,糖尿病合并冠心病组（50例,其中男性25例,女性25例）,正常对照组（50名,其中男性25名,女性25名）.收集24 h尿,通过竞争酶联免疫吸附法定量检测尿液中8-OHdG含量,苦味酸法测定尿肌酐浓度,采用酶法测定血脂、血糖、肾功能,高效液相法测定糖化血红蛋白（HbA1c）,Clauss法测定纤维蛋白原（FIB）.结果 与正常对照组[（6.97±2.13） ng/mgCr]相比,糖尿病组[（12.26 ±3.57） ng/mgCr]及糖尿病合并冠心病组[（14.41±2.84） ng/mgCr]尿8-OHdG水平较高,同时糖尿病合并冠心病组显著高于糖尿病组（P均＞0.05）.糖尿病及糖尿病合并冠心病组收缩压、腰围、体重指数、HbA1c、空腹血糖、负荷后2h血糖、甘油三酯、FIB水平显著高于正常对照组（P均＜0.01）,高密度脂蛋白-胆固醇水平低于正常对照组（P＜0.05）;糖尿病合并冠心病组HbA1c、负荷后2h血糖、FIB水平显著高于糖尿病组（P均＜0.01）;尿8-OHdG水平与收缩压、腰围、体重指数、甘油三酯、FIB、HbA1c、空腹血糖、负荷后2h血糖呈正相关;经多元逐步回归分析,HbA1c对尿8-OHdG水平有显著性影响（P ＜0.001）.结论 糖尿病及合并冠心病患者尿8-OHdG水平升高;尿8-OHdG可能成为评价糖尿病性动脉粥样硬化程度的新指标.
Objective To study the level of urinary 8-hydroxy deoxyguanosine （8-OHdG） in type 2 diabetic patients with or without coronary heart disease.Methods 150 cases were selected from hospitalized patients and the physical examination center in the General Hospital of Tianjin Medical University and were divided into diabetic group （n =50,male 25,female 25）,diabetic patients with coronary heart disease group （n =50,male 25,female 25） and healthy control group （n =50,male 25,female 25）.24 hours urine were collected,then urinary 8-OHdG was tested with enzyme linked immunosorbent assay,and urine creatinine was tested with trinitrophenol method.Blood glucose,creatinine,uric acid and blood urea nitrogen with enzymatic method,and HbA1 c with HPLC method,and fibrinogen （FIB） with Clauss method were also tested.Results Compared with normal control group [（6.97 ± 2.13） ng/mgCr],urinary 8-OHdG was increased in diabetes group [（12.26 ± 3.57） ng/mgCr] and diabetic coronary heart disease group [（14.41 ± 2.84） ng/mgCr].While compared with diabetes group,urinary 8-OHdG was significantly elevated in diabetic coronary heart disease group.Compared with healthy control group,the level of systolic blood pressure,body mass index,HbAlc,fasting blood glucose,2 hour postprandial blood glucose,triglyceride and fibrinogen were significantly higher in diabetic group and diabetic coronary heart disease group （all P 〈 0.01）,and the level of high density lipoprotein-cholesterol was lower （P 〈 0.05）.Compared with diabetes group,the level of HbA1c,2 h postprandial blood glucose and fibrinogen were significantly higher in diabetic coronary heart disease group （all P 〈 0.01）.Urinary 8-OHdG was positively related to systolic blood pressure,waist circumference,body mass index,triglyceride,fibrinogen,HbA1 c,fasting plasma glucose and 2 h postprandial blood glucose.After stepwise regressive analysis,HbA1c had significant effect on the levels of urinary 8-OHdG （P 〈 0.001）.Conclusions The level of urinary 8-OHdG is increased in patients with type 2 diabetes mellitus and diabetic patients with coronary heart disease.Urinary 8-OHdG might be a new marker for evaluating diabetic atherosclerosis.
出处
《国际内分泌代谢杂志》
北大核心
2014年第5期295-299,共5页
International Journal of Endocrinology and Metabolism
关键词
8-羟基脱氧鸟苷
糖尿病
冠心病
动脉粥样硬化
氧化应激
8-Hydroxy-2＆#39
-deoxyguanosine
Diabetes mellitus
Coronary heart disease
Atherosclerosis
Oxidative stress
