摘要
从中国云南省野外采集的梨花迁粉蝶(Catopsilia pyranthe)中分离得到一株疑似微孢子虫,对该分离株的核糖体SSUrRNA(Small subunit ribosomal RNA)和α-tubulin基因进行克隆测序。通过序列分析及系统进化树构建,结果发现该分离株属于Nosema属的一种微孢子虫,命名为Nosema sp.CP。通过对SSUrRNA系统进化分析发现,Nosema sp.CP与家蚕微孢子虫(Nosema bombycis)、斜纹夜蛾微孢子虫(Nosema spodopterae)的亲缘关系相对于菜粉蝶微孢子虫Nosema sp.MPr更加接近。通过对α-tubulin基因的系统进化分析,结果表明Nosema sp.CP与N.bombycis聚在一支上,进一步证实了它们的紧密关系。因此,本研究首次在粉蝶科(Pieridae)的梨花迁粉蝶中分离得到的Nosema属微孢子虫是与家蚕微孢子虫非常近源的一类微孢子虫,并暗示了粉蝶科昆虫感染的微孢子虫具有潜在多样性。
In this study, a new sample of Microsporidia was firstly isolated from mottled emigrant, Catopsilia pyranthe, in Yunan Mengzi of China. The sequences of small subunit ribosomal RNA （SSUrRNA） and a-tubulin gene were hoth sequenced to character- ize the phylogeny of this new isolate, designed as Noserna sp. CP. Phylogenetic analysis of SSUrRNA found that the isolate belong to the Nosema clade mainly comprised of Nosema bombycis, Nosema spodopterae, and is more distantly related to that of Nosema sp. MPr. a-tubulin tree further confirm that Nosema sp. CP was sistered to that of N. bombycis. This is the first report of Nosema species exist in Catopsilia pyranthe which shows close relationship to Nosema bombycis, indicative of species diversity of Nosema in Pieridae insects.
出处
《重庆师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第5期42-47,共6页
Journal of Chongqing Normal University:Natural Science
基金
国家高技术研究发展计划(863计划)子课题(No.2013AA102507)
重庆市自然科学基金计划项目(No.cstc2011jjA80003)
重庆市教委科学技术项目(No.KJ110611)
