摘要
目的探讨乳腺癌石蜡肿瘤组织和相应血液细胞中乳腺癌易感基因1(BRCA1)与切除修复交叉互补基因1(ERCC1)及胸苷酸合成酶(TS)基因表达的相关性,同时探讨BRCA1、ERCC1、TS在乳腺癌肿瘤组织与对应患者血液细胞中表达的关系。方法收集53例乳腺癌肿瘤石蜡标本及其对应的血液样本,利用实时荧光定量PCR技术检测肿瘤石蜡组织和对应血液样本中BRCA1、ERCC1、TS mRNA的表达水平;利用Pearson相关性分析方法分析肿瘤石蜡组织和相应血液细胞BRCA1、ERCC1、TS mRNA表达的相关性以及肿瘤石蜡组织细胞BRCA1、ERCC1、TS mRNA之间表达的相关性。结果乳腺癌石蜡组织中BRCA1 mRNA表达的ΔCT为(7.516±2.257),对应的血液组织中为(10.374±2.519)。乳腺癌石蜡组织中ERCC1和TS mRNA表达的ΔCT分别为(6.114±2.944)、(5.950±2.604),对应的血液组织分别为(8.801±2.581)、(10.078±1.731)。Pearson相关性分析显示,BRCA1、ERCC1在肿瘤石蜡组织与血液组织中的表达均呈正相关(r=0.607、0.537,P<0.05)。肿瘤石蜡组织与血液组织中的TS表达无相关性(r=0.074,P>0.05)。BRCA1与ERCC1在肿瘤石蜡组织中的表达无相关性(r=0.250,P>0.05)。BRCA1与TS在肿瘤石蜡组织中的表达无相关性(r=0.256,P>0.05)。ERCC1与TS在肿瘤石蜡组织中的表达无相关性(r=0.169,P>0.05)。结论乳腺癌患者的血液标本也许可以替代其肿瘤石蜡组织检测BRCA1和ERCC1 mRNA的表达;BRCA1与ERCC1、TS的表达无相关性。
Objective To investigate the correlation between the expression of breast cancer gene 1 （BRCA1）,excision repair cross-complementing gene 1（ERCC1） and thymidylate synthetase （TS） gene in paraffin tumor tissue and corresponding blood cells for breast cancer. Methods Tumor paraffin specimens and their corresponding blood samples in 53 cases were collected,the expression level of BRCA1,ERCC1,TSmRNA of tumor paraffin samples and corresponding blood samples were detected by real-time fluorescence quantitative PCR technology,Pearson correlation analysis method was used to analyze the correlation of BRCA1,ERCC1,TS mRNA expression of tumor paraffin tissue and the corresponding blood cells and the correlation of the expression between BRCA1,ERCC1,TS mRNA in the tumor paraffin tissue cells. Results In the breast cancer paraffin tissues,the △CT of BRCA1 mRNA expression was （7.516±2.257）,while in the corresponding blood tissue,the △CT was （10.374±2.519）.In the breast cancer paraffin tissue,the △CT of ERCC1 and TS mRNA expression was （6.114±2.944）,（5.950±2.604） respectively,while in the corresponding blood tissue,the △CT was （8.801±2.581）,（10.078±1.731） respectively.Pearson correlation analysis showed that there was positive correlation between the expression of BRCA1,ERCC1 in tumor paraffin tissue and blood tissue （r=0.607,0.537,P〈0.05）.There was no correlation between the expression of TS in tumor paraffin tissue and blood tissue （r=0.074,P〉0.05）.There was no correlation between BRCA1 and ERCC1,BRCA1 and TS,ERCC1 and TS mRNA expression in tumor tissue （r=0.250,r=0.256, r=0.169,P〉0.05）. Conclusion Blood samples of patients with breast cancer may be able to replace tumor paraffin tissue to detect the BRCA1 and ERCC1 mRNA expression.There was no correlation between the expression of BRCA1 andERCC1,TS in breast tumor tissue.
出处
《中国当代医药》
2014年第27期7-10,15,共5页
China Modern Medicine
