摘要
考察15个秀珍菇（Pleurotus geesterani ）菌株的主要农艺性状,并从14个引物中筛选出7个 ISSR 引物对其基因组DNA进行 PCR 扩增,分析其 DNA序列多态性。结果表明,参试秀珍菇 P02、P03、P04号菌株原基形成时间最短,接种34 d后开始现蕾；“农秀1号”最适出菇温度为20＆#177;2.0℃,P05、P14可以在较高温度出菇；“农秀1号”、P02、P03、P04产量较高。参试秀珍菇菌株遗传相似系数为0.66~1.00；采用UPGMA分析表明,在0.87处可将这15个秀珍菇菌株分成3类。
Selected agronomic characteristics of 1 5 Pleurotus geesterani strains sourced from different Chinese provinces were compared.Primordium formation by strains P02,P03,P04 occurred within the shortest time period （34 d）,and these strains,together with the in-house bred strain “nongxiu 1”,also produced the highest fruit body yields.Optimal fruiting temperatures varied between 19 ℃ （P12）and 25 ℃ （P05,P14）;five strains （P06,P07,P13,P15 and “nongxiu 1”）had an optimal fruiting temperature of 20 ℃,Strains were differentiated on the basis of ISSR markers generated using seven of 14 pre-selected primers.Genetic similarity coefficients （GSC）varied between 0.66~1.00,and the 15 strains formed three groups at a GSC level of 0.87.
出处
《食用菌学报》
北大核心
2014年第2期14-18,共5页
Acta Edulis Fungi
基金
浙江省农业新品种选育重大科技专项(编号:2012C12911)
国家"973"计划项目(编号:2014CB138300)
浙江省食用菌创新团队(编号:2009R50029)
浙江省重大科技专项(编号:2007C12002)的部分研究内容
关键词
秀珍菇
农艺性状
ISSR
Pleurotus geesterani
agronomic characteristics
ISSR
