摘要
通过硅胶柱层析法、Sephadex LH-20凝胶柱层析法和重结晶法,分离纯化药用拟层孔菌(Fomitopsis officinalis)子实体中的化学成分,依据化合物的物理性质,运用核磁共振谱(nuclear magnetic resonance,NMR)、质谱(mass spectrometry,MS)等波谱法分析鉴定化合物结构,并选用得率较高的4种化合物进行体外抗肿瘤实验。共分离出8个化合物,为4,6,8(14),22(23)-四烯-3-酮-麦角甾烷、麦角甾-7,22-二稀-3β-醇、3-酮基-去氢硫色多孔菌酸、去氢齿孔酸、阿里红酸A、去氢齿孔酮酸、阿里红酸C、阿里红酸B。其中化合物4,6,8(14),22(23)-四烯-3-酮-麦角甾烷为首次从药用拟层孔菌子实体中分离得到。阿里红酸A、阿里红酸C、3-酮基-去氢硫色多孔菌酸抑制人乳腺癌细胞MCF-7活性较好,阿里红酸A、阿里红酸C抑制人肝癌细胞SMMC-7721活性较好。
Eight compounds,isolated and purified from Fomitopsis officinalis fruit bodies by silica gel column chromatography,Sephadex LH-20 column chromatography and recrystallization,were identified by NMR and mass spectrometry as 4,6,8 （14）,22 （23）-tetraen-3-one-ergos-tane,ergosta-7,22,dien-3β-ol,3-keto-dehydrosulfurenic acid,dehydroeburicoic acid,dehydroeburiconic acid,and fomefficinic acids A,B and C. 4,6,8 （14）,22 （23）-tetraen-3-one-ergostane has been isolated from F.officinalis fruit bodies for the first time.Fomefficinic acids A and C,3-keto-dehydrosulfurenic acid and dehydroeburicoic acid were obtained in high yield and tested for anti-proliferative activity against two human cancer cell lines.Fomefficinic acids A and C,and 3-keto-dehydrosulfurenic acid,inhibited the growth of MCF-7 human breast cancer cells,while fomefficinic acids A and C inhibited the growth of SMMC-7721 human liver cancer cells.
出处
《食用菌学报》
北大核心
2014年第2期72-77,共6页
Acta Edulis Fungi
基金
国家自然科学基金(编号:31270088)的部分研究内容
关键词
药用拟层孔菌
化学成分
抗肿瘤
Fomitopsis officinalis
chemical composition
anti-proliferative activity
