期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

TRPV1与μ-阿片受体在胃投射脊髓背根神经节中的共存表达及其临床意义 被引量：1

The coexistence expression of TRPV1 and μ-opoid receptor in spinal dorsal root ganglion projected to stomach and its clinical significance
下载PDF
导出
摘要 目的:观察瞬时受体电位离子通道(TRPV1)与μ-阿片受体在胃调控脊髓背根神经节中的共存表达,探讨其在急性胃黏膜损伤治疗中的临床意义。方法:选取雄性Wistar大鼠分三组,正常对照组(NC组)、浸水束缚应激(WIRS)组(WIRS组)和舒芬太尼预处理组(SP组)。采用经典WIRS方法复制急性胃黏膜损伤(AGML)模型,于应激6 h后观察大体胃黏膜损伤程度,并记录溃疡指数(GI),并采用免疫荧光方法观察TRPV1与μ-阿片受体在胸段DRG神经元中的表达情况,并通过ELASA法测定各组大鼠胃黏膜组织中CGRP含量。结果:TRPV1与μ-阿片受体在胸段胃投射DRG神经元上共存表达,且AGML大鼠胃黏膜GI和CGRP含量都明显增高,而舒芬太尼预处理虽使CGRP含量更高,GI较WIRS组显现非常显著下降。结论:TRPV1参与了急性胃黏膜损伤的保护机制,激活μ-阿片受体可活化TRPV1释放CGRP从而发挥胃黏膜保护作用。 Objective To observe the coexistence expression of TRPV1 and μ-opoid receptorin spinaldorsal root ganglion （DRG） projected to stomach, and to investigate the relationship between TRPV1 andp.-opoid receptorand its clinical significance in rats with acute gastric mucosal lesion induced by water immersionand restraint stress. Methods FortyWistar rats were randomly assigned to 3 groups, including normal controlgroup（group NC, n = 10）, WIRS group （group WIRS, n = 20）, and sufentanil pretreatment group （group SP,n = 10）. A rat model of gsatric mucosal lesion was induced by WIRS. 6 hours after WIRS treatment, gastrictissues were excised and microscopically observed; ulcer index （GI） was noted. The coexistence expression ofTRPV1 and μ-opioid receptor in DRG neurons was detected by immunofluorescence assay, and the levels ofCGRP was measured by ELASA. Results As compared withgroup WIRS, the degree of gastric injury wasobviously relieved in group SF. Coexistence of TRPV1 and μ-opioid receptor was detected in thoracic DRGneurons projected to stomach; the CGRP level was higher in group WIRS than in group NC. ConclusionsTRPV1isinvolved in protection of acute gastric mucosal lesion. Activation of Ix-opioid receptor can induce TRPV1 torelease CGRP, resulting in protection of gastric mucosa.
作者 蒋群 屠伟峰 徐少群 赵高峰 郄文斌 李国才
机构地区 广东省中医院麻醉科 广州军区广州总医院全军临床麻醉中心
出处 《实用医学杂志》 CAS 北大核心 2014年第18期2876-2879,共4页 The Journal of Practical Medicine
基金 国家自然科学基金(编号:81272141) 广东省自然科学基金(编号:S2011010003373) 江苏省高校重点实验室开放课题(编号:KJS09002)
关键词 Μ-阿片受体 TRPV1 CGRP 舒芬太尼 急性胃黏膜损伤 ix-opioid receptor TRPV1 CGRP Sufentanil Acut gastric mucosal lesion
分类号 R614 [医药卫生—麻醉学]
  • 相关文献

参考文献11

  • 1Holzer P. Efferent-like roles of afferent neurons in the gut: Blood flow regulation and tissue protection [J]. Autonomic Neuroscience, 2006,125 (1) : 70-75.
  • 2Gyires K, Lner MU. Activation of central opioid receptors may induce gastric mucosal defence in the rat [J] . Physiology , 2001,95(1-6):189-196.
  • 3蒋群,屠伟峰.舒芬太尼预处理对大鼠急性胃粘膜病变的保护作用及与酸敏感离子通道的关系[J].南方医科大学学报,2010,30(5):1099-1102. 被引量：11
  • 4Geeraerts K, Mimidis L. Role of endogenous opioids in the control of gastric sensorimotor function [J]. Neurogastroenterol Motil, 2008,20(10): 1094-1102.
  • 5yires K, Ronal AK. Supraspinal delta- and mu-opioid receptors mediate gastric mucosal protection in the rat [J]. Pharmacol Exp Ther, 2001,297(3):1010-1015.
  • 6Allen A, Flemstrom G. Gastroduodenal mucus bicarbonate barrier: protection agaist acid and pepsin [J]. Physiol Cell Physiol, 2005,288(1): C1-C19.
  • 7Holzer P. TRPV J and the gut: from a tasty receptor for a painful vanilloid to a key player in hyperalgesia [J] . Pharmacol, 2004, 500 (1 - 3) : 231- 241.
  • 8Boillat A, Alijevic O. Calcium entry via TRPVl but not ASICs induces neuropeptide release from sensory neurons [J]. Mol CeliNeurosci, 2014,61(30): 13-22.
  • 9蒋群,屠伟峰,杨垂勋,李成龙.阿米洛利预处理对浸水束缚应激模型大鼠胃黏膜损伤的影响[J].实用医学杂志,2010,26(11):1926-1929. 被引量：4
  • 10Paul A, Shaaban A, Mousa, et al. u-Opioid Receptor Activation Modulaes Transient ReceptorPotential Vanilloid 1 (TRPV1) Currents in Sensory Neurons in A Model of Inflammatory Pain[J], Mol Pharmacol, 2007,71(1):12-18.

二级参考文献17

  • 1Holzer P. Taste receptors in the gastrointestinal tract [J]. Am J Physiol Gastrointest Liver Physiol, 2007, 292: 699-705.
  • 2Waldmann R. Proton-gated cation channels-neuronal acid sensors in the central and peripheral nervous system [J]. Adv Exp Med Biol, 2001, 502: 293-304.
  • 3Guth PH, Aures D, Panlsen G. Topical aspirin plus HCI gastric lesions in the rat[J]. Gastroenterology, 1979, 76(1): 88-93.
  • 4Gyires K, Ronal AK. Supraspinal - and -opioid receptors mediate gastric mucosal protection in the rat [J]. J Pharmacol Exp Ther, 2001, 297(3): 1010-5.
  • 5Holzer P, Wultsch T, Painsipp E, et al. Involvement of the acidsensing ion channel ASIC3, but not ASIC2, in acid hypersensitivity associated with gastritis in mice [J]. Gastroenterology, 2006, 130 (suppl 2): A250.
  • 6Holzer P. Acid-sensitive ion channels in gastrointestinal function[ J ]. Curr Opin Pharmacol, 2003, 3:618-25.
  • 7Kress M, Waldmann R. Acid sensing ionic channels[J]. Curt Top Membranes, 2006, 57: 241-76.
  • 8Vetter I, Bruce DW, Gregory RM, et al. The μ opioid agonist morphine modulates potentiation of capsaicin-evoked TRPVI responses through a cyclic AMP-dependent protein kinase A pathway[J]. Mol Pain, 2006, 2: 22.
  • 9Ugawa S, Ueda T, Yamamura H, et al. In situ hybridization evidence for the coexistence of ASIC and TRPVI within rat single sensory neurons[J]. Mol Brain Res, 2005, 136(1-2): 125-33.
  • 10Holzer P. Taste Receptors in the Gastrointestinal Tract.V. Acid sensing in the gastrointestinal tract [J]. Am J Physiol Gastrointest Liver Physiol, 2007,292 (3) : G699-705.

共引文献12

同被引文献11

  • 1Holzer P. Taste receptors in the gastrointestinal tract. V. Acid sensing in thegastrointestinal tract[J]. Am J Physiol Gastrointest Liver Physiol, 2007, 292(3 ) : G699-705.
  • 2Guth PH,Aures D, Panlsen G, et al. Topical aspirin plus HC1 gastric lesions in the rat. Cytoprotective effect of prostaglandin, cimetidine, and proban- thin[J]. Gastroenterology, 1979,76 ( 1 ) : 88-93.
  • 3Gautam M, Benson CJ. Acid-sensing ion channels (ASICs) in mouse ske- letal muscle afferents are heteromers composed of ASICla,ASIC2,and ASIC3 subunits[J]. FASEB J, 2013,27 (2) : 793-802.
  • 4Page AJ, Brierley SM, Martin CM, et al. Different contributions of ASIC channels la,2,and 3 in gastrointestinal mechanosensory function [J]. Gut,2005,54(10) : 1408-1415.
  • 5Vetter I, Wyse BD, Monteith GR, et al. The mu opioid agonist morphine modulates potentiation of capsaicin- evoked TRPV1 responsesthrough a cyclic AMP-dependent protein kinase A pathway[J]. Mol Pain,2006,2:22.
  • 6Harada N, Okajima K, Uchiba M, et al. Contribution of capsaicin-sensitive sensory neurons to stress-induced increases in gastric tissue levels of pro- staglandins in rats[J]. Am J Physiol Gastrointest Liver Physiol, 2003,285 (6) :G1214-1224.
  • 7Groneberg D, Lies B, K~nig P, et al. Cell-specific deletion of nitric oxide- sensitive guanylyl cyclase reveals a dual pathway for nitrergic neuromus- cular transmission in the murinefundus[J]. Gastroenterology,2013,145 ( 1 ) : 188-196.
  • 8Wultsch T, Painsipp E, Shahbazian A, et al. Deletion of the acid-sensing ion channel ASIC3 prevents gastritis-induced acid hyperresponsiveness of the stomach-brainstem axis[J]. Pain, 2008,134 (3) : 245-253.
  • 9Holzer P. Efferent-like roles of afferent neurons in the gut : blood flow re- gulation andtissue protection[J]. Auton Neurosci ,2006,125 (1/2) :70-75.
  • 10Li WF,Hao DJ,Fan T,et al. Protective effect of chelerythrine against ethanol- induced gastric ulcer in mice[J]. Chem Biol Interact ,2014,208 : 18-27.

引证文献1

实用医学杂志
2014年 第18期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部