摘要
目的:建立麝香接骨胶囊中松香酸的检测方法。方法:采用高效液相色谱法进行研究,色谱柱为VPODS(150mm×4.6mm,5μm);流动相为乙腈-10%甲醇的0.1%磷酸溶液(66∶34);检测波长:241nm;流速:1.0mL/min;柱温:30℃。结果:色谱分离度良好,制订出麝香接骨胶囊中松香酸的检测方法及检出限,松香酸在0.0747-0.4980μg,r=1(n=5)范围内呈良好的线性关系,平均加样回收率为96.68%,RSD为0.94%,检出限为0.021mg/g。结论:该方法准确、灵敏度高,重现性好,可用于麝香接骨胶囊中松香酸的检测控制。
Objective： To establish a method for the inspection of abietic acid in Shexiang Jiegu Capsule. Methods： The contents of cbietic acid in this preparation were measured by HPLC. VP-ODS （150mm×4.6mm, 5μm） column was used as the stationary phase, acetonitril-10% methanol 0.1% phosphate acid solution （66：34） as the mobile phase, with detection wavelengthat 241nm and the flow rate was 1.0mL/min, the column temperature was set at 30℃. Results： For the cbietic acid in Shexiang Jiegu Capsule, the resolution of the peaks was good. Quantitative analysis of HPLC showed that the linear ranges of cbietic acid was 0.0747-0.49801ag, r=1 （n=5）. The average recoveries of cbietic acid was 96.68%, RSD 0.94%, the detection limit was 0.021mg/g. Conclusion： This method is reliable and accurate.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2014年第9期2982-2984,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
