摘要
[目的]用高效液相色谱法（HPLC）考察5个不同产地女贞叶中熊果酸的含量.[方法]用高效液相色谱法所用色谱柱为十八烷基硅烷键合硅胶色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-甲醇-0.5％醋酸铵溶液（67∶12∶21）,检测波长为210 nm.[结果]方法学考察结果,回归方程为Y=4.715 5X＋ 17.918,R2 =0.999 4,熊果酸含量在5.21～125.12 μg/ml浓度范围内线性关系良好.用该方法测得5个不同产地女贞叶中熊果酸的含量存在差异,其中上海市产的含量最高,为2.157 4 mg/g,南阳唐河产的含量最低,为0.863 0mg/g.[结论]同一采集时间,不同产地的女贞叶因环境因素,所含熊果酸的含量差异较大.
[Objective]The content of ursolic acid in folium Ligustrum lucidus from different habitats was investigated by HPLC.[Method]Chromatographic column is eighteen alkyl silane bonded silica gel column chromatography （4.6 mm × 250 mm,5 μm）,the mobile phase is acetonitrile-methanol-0.5 ％ ammonium acetate （67∶12∶21）,the detection wavelength is 210 nm.[Result]Methodological study results：the regression equation：Y =4.715 5X ＋ 17.918,R2 =0.999 4,the content of ursolic acid in 5.21-125.12 μg/ml,the concentration of the linear relationship is good.The content of ursolic acid in folium Ligustrum lucidus from different habitats existis differences,the highest content of Shanghai produced is up to 2.157 4 mg/g,the lowest content produced in Nanyang Tanghe is 0.863 0 mg/g.[Conclusion]At the same acquisition time,the content of ursolic acid in folim Ligustrum lucidus from different habitats has significant differences because of environmental factors.
出处
《安徽农业科学》
CAS
2014年第28期9738-9739,共2页
Journal of Anhui Agricultural Sciences
基金
黄河科技学院校级立项(KYZR201311)
关键词
女贞叶
熊果酸
HPLC
含量测定
Ligustrum lucidus
Ursolic acid
HPLC
Content determination
