摘要
为了鉴定马铃薯引进品种和变异材料的遗传背景和亲缘关系,利用9对SSR引物对12个引进品种、12个费乌瑞它自然和辐射变异材料进行遗传多样性分析。结果表明:对24份马铃薯参试材料的DNA进行PCR扩增共获得178条扩增条带,其中167个为多态性条带,多态性比率为93.8%,平均每对引物扩增条带数为18.6条。多态性条带中有84.4%的条带大小为120~950bp。24份参试材料的遗传相似性系数在0.479~1.000。聚类分析显示,在遗传相似系数为0.65处,参试材料可明显分为4个类群,全部变异材料均聚在1个类群,品种间的多态性和遗传距离大于变异材料。
In order to identify the genetic background and relationship of the introduced varieties and mutation materials in potato, the genetic diversity of 12 introduced varieties and 12 natural and radial mutation materials of potato variety ‘ Favorita’ were analyzed by 9 pairs SSR markers. Results： There were 178 amplified bands, 167 polymorphism bands of which were obtained with a polymorphism rate of 93.8 %. The average of each SSR primer amplified was 18.6 bands. There were 84.4 % of polymorphism bands located in 120 -950 bp. The genetic similarity coe^cients of the 24 experimental materials were from 0. 479 to 1. 000. The UPGMA cluster analysis showed that all experimental materials could be obviously divided into four grups at the genetic similarity codHcient of 0.65. All mutation materials were gathered in the same group. The polymorphism and genetic distances of varieties were greater than the mutation materials.
出处
《西南农业学报》
CSCD
北大核心
2014年第4期1399-1403,共5页
Southwest China Journal of Agricultural Sciences
基金
贵州省科技攻关项目"马铃薯诱变新品系选育及变异基因的分子检测研究"[黔科合NY字(2011)3021]
贵州省农委动植物专项"表型及分子标记鉴定筛选马铃薯诱变新材料的研究"[黔农育专字(2011)029]
贵州省科技计划项目"贵州薯类育种及产业化研发平台建设[黔科创能院所(2010)4006]
贵州省农业科学院专项"转基因马铃薯的鉴定和安全性评估"[黔农科院专项(2009)002]
贵州省农科院研究生创新基金"马铃薯四倍体栽培种与二倍体野生种原生质体融合研究"[黔农科合(创新基金)2010012]
贵州省自然科学技术基金"贵州魔芋种质资源遗传多样性SRAP标记分析"黔科合J字(2013)2189]
