摘要
通过氢键调节作用建立一种快速分离与鉴定人工色素的薄层色谱法。以石油醚-二氯甲烷(7:3,V/V)为基础展开体系,分别对硅胶进行接枝改性和在基础展开体系中添加氢键调节剂,再对人工色素进行薄层色谱检测分析。结果表明:相较于硅胶,改性硅胶的氢键密度明显增大,其氢键密度随着改性剂质量浓度的增加而增大;主链型改性剂和氢键调节剂的引入增加了固定相、流动相与人工色素间形成氢键的可能性,改变了人工色素的Rf值。
This article establishes a thin layer chromatography（ TLC） for the fast separation and identification of synthetic pigment by hydrogen-bonding effects. Based on the conditions of petroleum ether- methylene chloride（ 7∶ 3,V /V）,TLC detection and analysis on synthetic pigment was carried out by grafting hydrogen-bonding modifier onto silica gel and adding hydrogen bonding regulator to based system respectively. The results suggest that hydrogen-bonding density of modified silica gel is significantly higher than that of silica gel and increases with modifiers mass concentration. Main chain modifier and hydrogen-bonding eluent increases the possibility of hydrogen-bonding between synthetic pigment and stationary phase,as well as mobile phase,which changes the Rfvalue effectively.
出处
《重庆理工大学学报（自然科学）》
CAS
2014年第8期55-59,共5页
Journal of Chongqing University of Technology：Natural Science
基金
重庆理工大学拔尖创新人才培养项目(BC201204)
关键词
人工色素
薄层色谱
氢键作用
synthetic pigment
thin layer chromatography
hydrogen-bonding effects
