摘要
目的:探讨鼻敏颗粒治疗变应性鼻炎(AR)的疗效机制。方法:将自由喂养1周大鼠,按雌雄随机分为4组,即正常对照组、肺气虚型AR模型组、鼻敏颗粒组、阳性药物辛芩颗粒组,每组15只。用刨花烟熏的基础上,以卵清蛋白致敏造成肺气虚型AR大鼠模型,予以鼻敏颗粒(10g·kg-1)、辛芩颗粒(10g·kg-1)治疗2周,采用免疫荧光法检测大鼠血清IL-4、IL-10的含量。结果:1)正常组与各组比较,血清IL-4和IL-10水平有显著差异(P<0.01),造模后血清IL-4和IL-10水平有明显升高;2)正常组、模型组在观察期前后比较,血清IL-4和IL-10水平无统计学差异(P>0.05),而辛芩颗粒组、鼻敏颗粒组干预前后比较,血清IL-4和IL-10水平差异有意义,(P<0.05),IL-4与IL-10水平均迅速下降,3)治疗后辛芩颗粒组与鼻敏颗粒组比较,IL-4与IL-10水平差异无显著性意义(P>0.05)。结论:鼻敏颗粒可降低肺气虚型AR大鼠血清IL-4、IL-10水平,可能是治疗肺气虚型AR的作用机制之一。
Objective： Discussing on effective mechanism of Bimin Granule in the treatment of allergic rhinitis （AR）. Methods： Free feeding rats for lweeks, according to sex were randomly divided into 4groups, namely controlled group, lung-Qi deficiency type AR model group, Bimin granule group, positive medicine Xinqin Granules group, with 15rats in each group. Based on the wood smoke, sensitized to ovalbumin caused lung-Qi deficiency type AR rat model, be allergic particles （ 10g. kg-1 ）, Xinqin Granule （ 10g. kg-1） treatment for 2weeks, were determined by immunofluorescence assay of serum IL-4, IL- 10in rats. Results： Compared with the normal group, serum IL-4and IL-10levels had significantly different （P〈0. 01 ）, after the model of serum IL-4and IL-101evels were significantly increased; 2） The normal group, model groups before and after the observation period, serum IL-4and IL-101evels and no significant difference （P〉0.05）, while the group Xinqin Granule before and after the intervention group Bimin Granule, serum IL-4and IL- 10levels and differences meaningful （P〈0. 05）, IL-4and IL-101evels were decreased rapidly; 3 ） After the treatment group compared with Xinqin Granule and Bimin Granule group. IL- 4and IL-101evel difference was no significant difference （P〉0.05）. Conclusion： Bimin Granule can reduce the serum IL-4, IL-101evels of lung-Qi deficiency type AR rats, may be the mechanism for the treatment of lung-Qi deficiency type of AR.
出处
《四川中医》
2014年第8期59-61,共3页
Journal of Sichuan of Traditional Chinese Medicine
基金
成都中医药大学校基金项目(编号:ZRYB200905)
