摘要
目的:为秦皮药材的进一步研究和开发提供科学依据。方法:利用水蒸气蒸馏法提取秦皮挥发油,采用气相色谱-质谱(GC-MS)联用技术对挥发油成分进行分析鉴定,用峰面积归一化法计算各成分的质量分数。结果:共分离出65个化学成分,鉴别出61个化学成分,占挥发油总量的93.968%。其中,质量分数较大的成分有4-己基-2,5-二氧代呋喃-3-乙酸(10.197%)、3-氟-4-甲氧基苯胺(9.846%)、葡萄螺烷(7.252%)、1-十五烯(7.228%)、右旋橙花叔醇(6.501%)、氧化芳樟醇(5.465%)、α-红没药醇(4.959%)、反-氧化芳樟醇(4.582%)、4-萜烯醇(3.203%)、柠檬烯(2.134%)、正癸酸(2.019%)。结论:GC-MS联用技术可快速、准确地鉴别秦皮挥发油成分,为秦皮资源的综合开发利用提供了基础数据。
OBJECTIVE：To provide scientific evidence for further RD of Cortex Fraxini. METHODS：The volatile oil was extracted from Cortex Fraxini by steam distillation. The volatile oil was analyzed by GC-MS and the mass fraction of each component was calculated by normalization method. RESULTS：65 chemical compounds were separated and 61 of them were identified,which accounted for 93.97% of all the volatile oil. The major components were 4-hexyl-2,5-dioxo furan-3-acetic acid（10.197）,3-fluoro-4-methoxyaniline（9.846）,vitis-pirane（7.252）,1-pentadecene（7.228%）,D-nerolidol（6.501%）,linalyl oxid（5.465%）,α-bisabolo（4.959%）,trans-linalool oxide（4.582%）,4- terpenol（3.203%）,limonene（2.134%）and n-decanoic acid（2.019%）.CONCLUSIONS：The volatile oils of Cortex Fraxini can be accurately identified by GC-MS rapidly,which provide basic data for comprehensive utilization of Cortex Fraxini.
出处
《中国药房》
CAS
CSCD
2014年第35期3310-3312,共3页
China Pharmacy
基金
贵州省科技计划课题(No.黔科合院所创能[2009]4010)
