摘要
目的：建立同时测定柴银口服液中绿原酸、葛根素和黄芩苷含量的方法。方法：采用高效液相色谱（HPLC）法。色谱柱为Purospher？STAR RP-18 endcapped（55 mm×4 mm,3μm）,流动相为甲醇-2%冰醋酸水溶液（梯度洗脱）,检测波长为327 nm（0-〈4 min）、250 nm（4-〈6 min）、280 nm（6-10 min）,流速为1.0 ml/min,柱温为30℃。结果：绿原酸、葛根素和黄芩苷的质量浓度分别在0.436 8-8.736 0μg/ml、0.206 4-4.128 0μg/ml和2.060-41.200μg/ml范围内与各自峰面积积分值呈良好的线性关系（r均为0.999 9）;三者精密度、稳定性、重复性试验的RSD〈3%;平均加样回收率分别为99.57%、99.91%和100.08%,RSD分别为1.75%、1.69%和2.20%（n均为6）。结论：波长切换HPLC法简便、快速、准确、可靠,可用于柴银口服液质量的全面控制与评价。
OBJECTIVE：To establish the method for the content determination of chlorgenic acid,puerarin and baicalin in Chaiyin oral solution. METHODS：HPLC method was adopted. The determination was performed on Purospher？STAR RP-18 endcapped（55 mm×4 mm,3 μm）column with mobile phase consisted of methanol-2% glacial acetic acid（gradient elution）at the flow rate of 1.0 ml/min. The detection wavelength was set at 327 nm（0-4 min）,250 nm（4-6 min）and 280 nm（6-10 min）. The column temperature was set at 30 ℃. RESULTS：The liner ranges of chlorgenic acid,puerarin and baicalin were 0.4368-8.736 0 μg/ml（r=0.999 9）,0.206 4-4.128 0 μg/ml（r=0.999 9）and 2.060-41.200 μg/ml（r=0.999 9）,respectively. RSDs of precision,stability and reproducibility tests were all lower than 3%. Average recoveries were 99.57%（RSD=1.75%,n=6）,99.91%（RSD=1.69%,n=6）and 100.08%（RSD=2.20%,n=6）,respectively. CONCLUSIONS：The method is simple,rapid,accurate and reliable,and it could be used for the quality control and evaluation of Chaiyin oral solution.
出处
《中国药房》
CAS
CSCD
2014年第35期3327-3329,共3页
China Pharmacy
