摘要
对橡胶树株产干胶等与胶乳产量相关性状进行了QTL分析。以热研88-13×IAN873杂交授粉子代F1群体为材料,测定其株产干胶、干胶含量、乳管列数、乳管个数和乳管大小等与橡胶树胶乳产量相关的性状,利用FsQtlMap 1.0软件,复合区间作图法(CIM,composite interval mapping),以已构建的SSR连锁图谱为基础进行QTL分析。结果显示,各性状偏度和峰度符合正态分布,除乳管大小外,各性状的平均值和方差有较大变化;株产干胶的变异系数最大,各因子的变异系数顺序为株产干胶>乳管个数>乳管列数>乳管大小>干胶含量;LOD阈值大于2.5,检测到与株产干胶相关的QTL 5个,分别可解释71.3%、69.1%、68.3%、64.4%和62.9%的群体变异,与乳管大小相关的QTL 3个,分别可解释84.5%、81.3%和15.4%的群体变异,未检测到与其他性状相关的QTL。
Objective of the study is about to detect quantitative trait loci（ QTLs） related to latex yield such as yield per plant and size of laticifer of rubber tree. F1 population derived from crosses of the rubber tree cultivars Reyan88-13 × IAN873 was used for QTL analysis. Data of trait about yield per plant,dry rubber content（ DRC）,line of laticifer,number of laticifer,and size of laticifer were collected and recorded. QTLs were detected by composite interval mapping（ CIM） using FsQtlMap 1. 0 based on the linkage map constructed with SSR markers. The results showed that the skewness and kurtosis of these traits were conformed to normal distribution except number of laticifer,the mean and variance of each trait had great changes. The variation coefficient of yield per plant was maximum,and the order was yield per plant〉 number of laticifer 〉line of laticifer 〉size of laticifer〉 dry rubber content. The presence of five main-effect QTLs which were related with yield per plant was detected when LOD threshold was greater than 2. 5,which could explain 71. 3%,69. 1%,68. 3%,64. 4%,and 62. 9% population variation respectively. Three main-effect QTLs which were related with number of laticifer was detected,which could explain 84. 5%,81. 3% and 15. 4% population variation respectively. There were no effect QTLs related with the other traits.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2014年第5期1060-1066,共7页
Journal of Plant Genetic Resources
基金
中央级公益性科研院所基本科研业务费项目(ITBBZD0715)
国家自然科学基金(31100495)
海南省自然科学基金(314084)
