摘要
目的:研究TGFβ(经典和非经典)通路在运动缓解衰老性肌肉流失(少肌症)中的作用。方法:24只雄性Wistar大鼠分为3组,C组(青年安静组)、S组(40dD-半乳糖注射致衰老组)、E组(40dD-半乳糖注射+6wk跑台运动的衰老运动组)。检测各组大鼠体重、腓肠肌重量、TGFβ(经典与非经典)通路因子——TGFβ1、MSTN、Phospho-smad2/3、PhosphoMAPKs(p38、JNK1/2、ERK1/2)及通路效应因子——p21、Pax7(WB法)、MyoD mRNA、MyoG mRNA(RT-PCR法)。结果:与C组相比,S组腓肠肌比重(腓肠肌重量/体重)降低,TGFβ1、MSTN、Phospho-smad2/3、Phospho-MAPKs(Phospho-p38、Phospho-JNK1/2、PhosphoERK1/2)、p21、MyoD mRNA、MyoG mRNA升高,Pax7降低;与S组相比,E组腓肠肌比重升高,TGFβ1、MSTN、Phospho-smad2/3、Phospho-MAPKs(Phospho-JNK1/2、Phospho-ERK1/2)降低,p21、Pax7、MyoD mRNA、MyoG mRNA升高。结论:运动训练抑制了TGFβ经典及非经典通路并缓解了衰老肌肉的流失,Pax7、p21、MyoD、MyoG可能作为TGFβ通路的效应器介导了该过程。
Objectvie：To explore the functions of（canonical and uncanonical）TGFβpathway in exercise-induced improvement of sarcopenia.Method：24 male wistar rats were divided into 3groups：C group（quiet young group）,S group（40day D-Glactose injection-induced senecence）,E group（40days D-Glactose injection-induced senecence＋6weeks exercise trainning on treadmill）.To detect body wet weight,gastrocnemius wet weight（by weighment）,TGFβpathway component-TGFβ1,MSTN,Phospho-smad2/3,Phospho-MAPK（p38,JNK1/2,ERK1/2）,TGFβpathway effector-p21,Pax7 by WB and MyoD mRNA,MyoG mRNA by RT-PCR in each group.Result：The ratio of gastrocnemius weight to body weight decreased,but TGFβ1,MSTN,Phospho-smad2,3,Phospho-p38,Phospho-JNK1,2,Phospho-ERK1,2,p21,MyoD mRNA,MyoG mRNA increased and Pax7 decreased in S group vs C group.The ratio of gastrocnemius weight to body weight increased,but TGFβ1,MSTN,Phospho-smad2,3,Phospho-JNK1,2,Phospho-ERK1,2decreased,Phospho-p38 unchanged,and p21,Pax7,MyoD mRNA,MyoG mRNA increased in E group vs S group.Conclusion：Exericse trainning can improve senescene-induced muscle loss by inhibiting TGFβcanonical and uncanonical pathway with the mediation of Pax7,p21,MyoD and MyoG.
出处
《体育科学》
CSSCI
北大核心
2014年第10期72-77,共6页
China Sport Science
基金
广东省教育厅科研项目(2012WYM_0126)
吉林省科技发展计划(20100593)
