摘要
目的探讨ROS1融合基因与切除修复交叉互补基因1(excision repair cross-complementation group 1,ERCC1)和核苷酸还原酶亚单位M1(ribonucleotide reductase subunit M1,RRM1)mRNA在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中表达的关系。方法应用实时荧光定量PCR方法检测257例NSCLC中ROS1基因及ERCC1和RRM1基因mRNA的表达。结果 ROS1融合基因及ERCC1和RRM1基因mRNA的表达均与患者性别、年龄、是否吸烟、肿瘤大小、淋巴结是否转移及临床分期无关(P>0.05)。ROS1融合基因与ERCC1和RRM1基因mRNA表达水平无关,且ERCC1基因mRNA表达水平与RRM1基因mRNA表达水平无关(P>0.05)。结论 NSCLC组织中ROS1融合基因表达可能与ERCC1和RRM1基因表达无关。
Objective To explore relationships between ROS1 fusion gene,excision repair cross complement protein 1 gene（excision repair cross-complementation group 1,ERCC1）and nucleotide reductase subunits M1（ribonu-cleotide reductase subunit M1,RRM1）of mRNA expressions in tissues of non small cell lung cancer（NSCLC）. Meth-ods Levels of ROS1 fusion gene and mRNA expressions of ERCC1 and RRM1 gene in 257 NSCLC patients were detected with real-time fluorescent quantitative PCR method. Results Levels of ROS1 fusion gene,and mRNA expressions of ERCC1 and RRM1 gene did not relate to gender,age,smoking history,tumor size,lymph node metastasis and clinical stage of patients（P〉0. 05）;expression level of ROS1 fusion gene did not relate to mRNA expressions of ERCC1 and RRM1 gene,and the expression level of ERCC1 mRNA did not relate to the expression level of RRM1 mRNA（ P 〉0. 05）. Conclusion Expression of ROS1 fusion gene in tissues of non small cell lung cancer may not relate to the ER-CC1 and RRM1 expressions.
出处
《解放军医药杂志》
CAS
2014年第9期1-4,共4页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
吴阶平医学基金会临床科研专项资助基金项目(320.6750.1360)
关键词
癌
非小细胞肺
ROS1
切除修复交叉互补基因1
核苷酸还原酶亚单位
M1
Carcinoma, non-small-cell lung
ROS1
Excision repair cross-complementation group 1
Ribonu- cleotide reductase subunit M1
