摘要
目的探讨丹参对体外培养大鼠成纤维样滑膜细胞增殖及bax、bcl-2表达的影响。方法取浓度为1.5×10-5个/ml的RSC-364成纤维样滑膜细胞悬液100μl加入细胞培养板,培养24 h,加入含不同浓度丹参的培养液,采用MTT法分析丹参对成纤维样滑膜细胞增殖抑制作用及其时效关系;免疫组化法检测bcl-2、bax表达情况。结果丹参可明显抑制大鼠成纤维样滑膜细胞增殖,并显示出一定的量效和时效关系。丹参可显著上调大鼠成纤维样滑膜细胞bax表达,下调bcl-2表达,与空白对照组比较差异有统计学意义(P<0.05)。结论丹参对体外培养大鼠成纤维样滑膜细胞的增殖具有抑制效应,其作用机制可能与调控bax、bcl-2表达有关。
Objective To explore the effect of Salvia Miltiorrhiza on fibroblastic synovial cells of bax/bcl-2 ex-pressions and proliferation culture in rats in vitro. Methods A concentration of 1. 5 ＆#215; 10 -5/ ml RSC-364 fibroblastic synovial cells of 100 ul cell suspension was added with the cell culture plate,and was cultivated for 24 h,and then differ-ent concentrations of Salvia Miltiorrhiza culture medium were added. Inhibition effect of Salvia Miltiorrhiza on fibroblastic synovial cell proliferation and its time-effect relationship were analyzed using MTT method;bcl-2 and bax expressions were detected using immunohistochemical method. Results Salvia Miltiorrhiza was able to inhibit the cell proliferation of fibroblastic synovial cells in rats,and it showed a certain degree of concentration-response and time-effect relationships. Salvia Miltiorrhiza could significantly increase the bax expression of fibroblastic synovial cells,and reduce bcl-2 expres-sion of synovial cells,and the differences,compared with those in control group,were statistically significant（ P 〈0. 05）. Conclusion Salvia Miltiorrhiza can inhibit the proliferation of fibroblastic synovial cell culture in rats in vitro , and its mechanism of action may relate to regulation of bax and bcl-2 expressions.
出处
《解放军医药杂志》
CAS
2014年第9期13-16,共4页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
江苏省自然科学基金资助项目(BK20131418)
江苏省高等学校大学生实践创新训练计划项目(2012JSSPITP1130)
关键词
丹参
成纤维细胞
细胞增殖
大鼠
Salvia miltiorrhiza
Fibroblasts
Cell proliferation
Rats
