摘要
以3年生短梗大参(Macropanax rosthornii)硬枝为插穗,分别用清水浸泡(对照)和500 mg/L IBA溶液浸泡,采用随机区组设计,扦插于黄土基质中,分析其生根特性,并测定生根过程中插穗的可溶性糖、可溶性蛋白质含量和多酚氧化酶(PPO)、过氧化物酶(POD)、吲哚乙酸氧化酶(IAAO)活性。以1年生嫩枝为材料,探寻短梗大参插穗生根的解剖学机理。结果表明:短梗大参在扦插生根过程中不产生愈伤组织,生根类型为皮部生根型,对照的扦插生根率高达97%;插穗的可溶性糖含量在根诱导和表达阶段均比扦插前降低,最大降幅达64.75%,而在根生长阶段升高;可溶性蛋白质含量在根诱导阶段比扦插前明显下降,最大降幅达69.18%,而在表达和生长阶段均比扦插前上升,最大增幅为55.51%;PPO活性在根诱导阶段比扦插前增加,最大增幅达515.85%,在表达阶段下降至最低,在生长阶段又上升,与扦插前相比,最大增幅达590.24%;POD活性在根诱导阶段和生长阶段均增加,与扦插前相比,最大增幅为59.20%;IAAO活性在根诱导阶段逐渐升高,在表达阶段达到峰值,比扦插前增加了14.84%,生长阶段则下降至扦插前水平。与对照相比,IBA处理可提高插穗不定根数量及生根过程中可溶性糖和蛋白质的含量,但PPO、POD和IAAO活性几乎不受外源IBA的影响。
Contrasted by water dipped samples, 3-year hard-wood cuttings ofMacropanax rosthornii, dipped with IBA at a concentration of 500 mg/L, were inserted into a substrate of yellow subsoil according to completely randomized block design. Rooting characters and the changes of soluble sugar content, soluble protein content and activity of polyphenol oxidase （PPO）, peroxidase （POD）, indoleacetic acid oxidase （IAAO） were studied during cutting rooting. The anatomic mechanism of rooting was also studied by using 1-year soft-wood cuttings of Macropanax rosthornii. The results showed that the new rooting originated from cortex with a 97% rooting rate, and there was no callus formation at the base. The soluble sugar content was decreased either in root-induced stage or in early production stage, and the maximal decrement was 64.75% compared with that of pre-cutting, while, it increased in root formation stage. The soluble protein content was decreased by 69.18% in root-induced stage and increased both in root formation stage and in root elongation stage, with a maximal increment of 55.51% compared with those of pre-cutting. The activity of PPO was increased to the maximum with a 515.85% increment in root-induced stage and decreased to the lowest in root formation stage, and then increased to the maximum with a 590.24% increment in the root elongation stage compared with those of pre-cutting. The activity of POD reached its peak either in root-induced stage or in root elongation stage with a maximal increment of 59.20% compared with that ofpre-cutting. The activity oflAAO was increased gradually in root-induced stage, it reached to a peak with an increment of 14.84% in root formation stage compared with that ofpre-cutting, and then decreased to the pre-cutting level in root elongation stage. The root number, sugar content and protein content of cuttings in the treatment of IBA were higher than those of in the control, while, the activity of PPO, POD and IAAO were hardly affected by the treatment of exogenous IBA.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第5期519-524,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省教育厅科学研究重点项目(11A130)
关键词
短梗大参
扦插
生根特性
营养物质含量
氧化酶活性
Macropanax rosthornii
cutting
rooting character
nutrients content
oxidase activity
