摘要
为分离和筛选芸芥自交亲和相关基因,以芸芥自交亲和系(SC)和自交不亲和系(SI)开花前及开花后的花药和柱头为试材,利用mRNA差异显示技术筛选芸芥自交亲和相关差异表达基因,结合实时荧光定量PCR技术验证差异表达基因在SI和SC开花前后不同时期的表达水平差异。结果共筛选得到了11条差异片段。差异表达基因包含木葡聚糖半乳糖基转移酶、DnaJ伴侣蛋白、果胶酸裂解酶家族蛋白、40S核糖体蛋白s19-1和假设蛋白等。木葡聚糖半乳糖基转移酶基因在SC开花前柱头中表达量最大,其表达量是SI开花前柱头中的4.41倍;SC8假设蛋白基因在芸芥SC开花后花药中表达量最大,是SI开花后花药中的23.98倍。初步推测这些差异基因可能在芸芥SI和SC性状调控过程中发挥重要作用。
To screen the genes associated with the self- compatibility of Eruca sativa,RNAs isolated from anther and stigma of pre- bloom and after flowering were used to investigate differential gene expression in SC and SI of E. sativa through DDRT- PCR( differential display reverse transcriptase polymerase chain reaction) method.The key differential genes before and after flowering in SI and SC were confirmed with real- time PCR. Results showed that 11 differential genes were obtained coding xyloglucan galactosyltransferase,chaperone protein dnaJ,pectate lyase family protein,40 S ribosomal protein s19- 1,hypothetical protein,etc. Xyloglucan galactosyltransferase gene in SC stigma had the highest expression before flowering and were 4. 41 folds than that in SI stigma.Hypothetical SC8 gene in SC anther had the highest expression after flowering and were 23. 98 folds than in SI anther. Results indicated that the above genes might have played important roles in SI and SC characteristics of regulation of E. sativa.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2014年第5期580-585,共6页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(3ZS061-A25-076)
现代农业产业技术体系建设专项(CARS-13)
甘肃省油菜工程技术研究中心项目(1306NTGA022)
关键词
芸芥
自交亲和基因
DDRT-PCR
表达分析
Eruca sativa
Self-compatibility gene
DDRT-PCR(differential display reverse transcriptase polymerase chain reaction)
Expression analysis
