摘要
目的:观察麻枳降浊方对便秘型肠易激综合征大鼠肠组织5-HT的影响,探讨其可能的作用机制及作用靶点。方法:将雄性wistar大鼠随机分为5组:正常组、模型组、西药组、中成药组和中药组,药物治疗组分别给予西沙比利、复方芦荟胶囊、麻枳降浊方,模型组给予蒸馏水灌胃,各组大鼠正常饲养,不限食水。采用HE染色法观察大鼠肠组织病理学形态,采用免疫组织化学法观察大鼠肠组织中5-HT表达水平。结果:与模型组相比,各治疗组小肠推进比明显增高(P<0.05),且肠道各组织5-HT表达水平显著下降(P<0.05)。结论:麻枳降浊方可能通过降低肠道各组织中5-HT的表达,从而改善肠道敏感性及动力异常,发挥对便秘型肠易激综合症大鼠的治疗作用。
Objective： To observe the influence of Mazhi Jiangzhuo formula on 5-HT in intestinal tissues of rats with constipationpredominant irritable bowel syndrome and explore its possible mechanism and target. Method： The male wistar rats were randomly dividedinto five groups： normal group, model group, western medicine group, Chinese patent medicine group and Chinese medicine group.The medicine groups were given cisapride, compound aloe capsule and Mazhi Jiangzhuo formula. The model group was givengastricperfusion of distilled water. The rats were normally feed, food and water free. The pathological histology of rats’ intestinal tissuewere observed by HE staining method. The expression level of 5-HT in the rats’ intestinal tissue was observed byImmunohistochemical technique. Result： Compared with the model group, the propulsion rate of small intestine of the treatment groupswere significantly increased （P〈0.05） and the expression level of 5-HT is significantly reduced （P〈0.05）. Conclusion： Mazhi Jiangzhuoformula improves the sensitivity and motility abnormalities of intestines probably by inducing the expression level of 5-HT in intestinaltissues. This is why it can treat constipation predominant irritable bowel syndrome.
出处
《中医临床研究》
2014年第30期35-37,共3页
Clinical Journal Of Chinese Medicine
基金
河北省科技支撑计划项目:麻枳降浊方改善便秘型肠易激综合征模型大鼠肠组织中5-HT
MC
ICC的实验研究
关键词
麻枳降浊方
便秘型肠易激综合征
5-HT
Mazhi Jiangzhuo formula
Constipation predominant irritable bowel syndrome
5-HT
