摘要
目的:研究重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)、纳米银对深Ⅱ°烫伤创面愈合过程炎症反应的影响。方法:用Wistar大鼠建立深Ⅱ°烫伤模型,分为A、B、C 3组,A组(30例):凡士林纱布覆盖;B组(30例):纳米银覆盖;C组(30例):rhGM-CSF涂抹创面。伤后第1,4,7,10,14,21天,观察创面炎症反应,细菌培养并计数,按照酶联免疫吸附法测定血清中白细胞介素-2(IL-2)、白细胞介素-8(IL-8)水平。结果:炎症反应,A组>B组>C组;A组、B组/C组分别于伤后第4、10天出现细菌生长,B组、C组与A组相比,细菌生长数量少,并且第10,14,21天差异有统计学意义(P<0.05),B组和C组之间差异无统计学意义(P>0.05);IL-2水平,伤后第1天各组间及第4天B组和C组之间差异无统计学意义(P>0.05),其余各时相点各组间差异有统计学意义(P<0.05),IL-8水平,伤后第1天,A组和B组、B组和C组之间无统计学意义(P>0.05),其余各时相点各组间差异均有统计学意义(P<0.05)。结论:rhGM-CSF、纳米银外用均能减少创面细菌生长,减轻炎症反应,并且rhGM-CSF在减轻创面炎症反应方面,更有优势。
Objective：To study the effect of recombinant human granulocyte/macrophage colony stimulating factor（rhGM-CSF）and nano-silver on deep burn degreeⅡ through observing the changes of inflammatory reaction during the healing process.Methods：The burn models were established on Wistar rats,and then were randomly divided into three groups according to petrolatum treatment（group A,n=30）,nano-silver treatment（group B,n=30）,and rhGM-CSF treatment（group C,n=30）,respectively.Bacteria samples were extracted from the wound for culturing and counting after 1,4,7,10,14,and 21day（s）respectively of treatment.The serum concentrations of interleukin-2（IL-2）and interleukin-8（IL-8）were measured in serum with ELISA.Results：The most severe inflammatory reaction was found in group A,while the mildest one wasfound in group C.Bacteria were found from the 4th day in group A and from the 10 th day in group B and group C after treatment,and the bacterial counting was less in group B and group C than in group A on the 10 th,14th,and 21 st days（P〈0.05）,but there were no significant difference between group B and group C in bacterial counting（P〉0.05）.Except on the 1st or4 th days,significant difference was found among different groups and different time points in serum IL-2and IL-8（P〈0.05）.Conclusion：rhGM-CSF and nano-silver could alleviate the inflammatory reaction after burn and inhibit the bacterial growth,but rhGM-CSF showed better effect than nano-silver.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2014年第5期674-677,共4页
Medical Journal of Wuhan University
基金
河北省2013年医学科学研究重点课题计划指令性课题(编号:20130024)
承德市科学技术研究与发展计划项目(编号:20122164)
