摘要
植物常见病原真菌尖孢镰刀菌(Fusarium oxysporum)为指示菌,采用平板扩散法对实验室保藏的38株极地细菌进行了抑菌活性验证,并对其中活性较强的菌株进行了分子鉴定与系统发育分析及次级代谢产物合成功能基因(PKS和NRPS)的克隆与分析。结果表明,38株极地细菌中,13株具有明显的抗病原真菌活性;分子鉴定与系统发育分析表明,11株活性菌株属于Pseudomonas,1株属于Psychrobacter,1株属于Arthrobacter;对活性菌株PKS和NRPS基因的克隆与分析表明,从10株活性菌株克隆到12条NRPS A结构域基因片段,其中5株活性菌株同时含有PKS I型KS结构域基因片段。据此为从聚酮和非核糖体肽等生物合成途径深入研究活性菌株的次级代谢产物提供了基因学证据。
The antifungal activity against plant pathogenic fungi Fusarium oxysporum of 38 strains of polar bacteria was validated by method of agar diffusion.The results showed that 13 strains had obvious antimicrobial activity.Molecular identification and phylogenetic analysis on the basis of 16S rRNA gene indicated that 11 strains belonged to genus of Pseudomonas and 2 strains belonged to genus of Psychrobacter and Arthrobacter respectively.The PKS and NRPS genes of these antifungal strains were also cloned and analyzed,which might be responsible for biosynthesis of bioactive secondary metabolites.The 12 gene fragments of the domain A of NRPS and the domain KS of type Ⅰ PKS were cloned from 10 and 5 antifungal strains,respectively.This paper provides a genetic evidence for deeply study these antifungal strains in which the PKS and NRPS biosynthetic pathways may applied.
出处
《海洋科学进展》
CAS
CSCD
北大核心
2014年第3期396-404,共9页
Advances in Marine Science
基金
海洋公益性行业科研专项经费项目--深远海(极地)微生物生物农药的研制与中试示范(201005032-2)
