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白僵菌菌株退化的酯酶同工酶比较分析 被引量:1

The esterase isozyme analysis of degenerate strains of Beauveria bassiana used to biocontrol Apriona germari
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摘要 白僵菌是桑天牛的生物防治菌种之一。为探究白僵菌菌种退化的遗传机理,以便利用生物工程技术控制菌种退化、提高其生物防治效率,采用酯酶同工酶技术分析各代白僵菌株在蛋白质水平的变异情况,结果表明:球孢白僵菌酯酶同工酶酶谱共出现2-7条酶带,随着传代次数的增加,其主要酶带一直存在且酶谱类型未变,但酶的活性在不断减弱,尤其是几条主要酶带(A、B、E、C )的活性变化更为明显;从聚类图中各菌株排列次序来看,各代菌株按照传代顺序排列,与第1代菌株比较,继代次数越多其间的类间距越大,变异越大,相似度越低,表现出一定的遗传多样性,严重影响其杀虫效果,说明酯酶同工酶技术可以在一定程度上反映白僵菌菌株的退化。 Beauveria bassiana was an important biocontrol strain .The study about the genetic mechanism of degeneration would contribute to the strain degradation control using biological engineering technology and the enhancement of biocontrol efficiency .In this study ,the vari-ation at the protein level of B .bassiana strains was alalyzed using esterase isozyme technolo-gy .The results showed that there are 2-7 isozyme belts ,and the main esterase isozyme belt also existes .Different subcultures show a same type of zymogram ,but its activity decreases obviously from generation to generation .Especially the activity change of a few main en-zymes is more apparent .The figure of clustering analysis shows that the generation ordering by passage .Compared with the 1st generation ,the genetic distance become longer from gen-eration to generation .In all ,more generation means more aberrance ,indicating that RAPD technology can reflect the strain degradation to some extent .beltwidth.
出处 《河北林果研究》 2014年第3期303-306,共4页 Hebei Journal of Forestry and Orchard Research
基金 河北省自然科学基金"白僵菌菌株退化的遗传学分析"(C2011204041) 河北农业大学青年科学基金"白僵菌菌株退化相关基因的RAPD和RFLP标记"(QJ201211)
关键词 白僵菌 酯酶同工酶 退化 Beauveria bassiana degeneration esterase isozyme
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