摘要
目的：速率散射比浊法测定抗环瓜氨酸肽抗体检测试剂盒的方法学评价。方法：对试剂盒的回收率、精密度、批间差、线性、特异性等进行验证。结果：抗环瓜氨酸肽抗体检测试剂盒的批内精密度为6%（低值）、4%（高值）,批间精密度为7%（低值）、6%（高值）,回收率为92%（低值）、104%（高值）,线性范围为0.2～9 mg/ml,线性方程为Y=1.0179X-0.0346,R^2=0.9960,抗干扰性强,血红蛋白≤5 g/L、甘油三酯≤5 mmol/L、胆红素≤97μmol/L对测定无影响。结论：抗环瓜氨酸肽抗体检测试剂盒检测样本快速、准确、可靠,适用于诊断。
Objective：To evaluate the performance of anti-cyclic cirullinated peptide antibodies kit system with rate nephelometry.Method：The withinrun and between-run percisions,linerity,correlation and reference range of anti-cyclic cirullinated peptide antibodies kit system was verified.Result：The within-run precision were 6%（low value）,4%（high value）,and the between-run precision were 7%（low value）,6%（high value）,the recovery were 92%（low value）,104%（high value）.The linearity range was 0.2-9 mg/ml,and the correlation equation was Y=1.0179X-0.0346,R^2=0.9960.Hemoglobin,triglyceride and bilirubin had rarely interfere.Conclusion：The method is single,rapid,stable,sensitive and accurate.It has the ability to anti-interference of hemoglobin,triglyceride and bilirubin.It can be used in the routine determination of anti-cycliccirullinated peptide antibodies.
出处
《中外医学研究》
2014年第28期148-150,共3页
CHINESE AND FOREIGN MEDICAL RESEARCH
关键词
抗环瓜氨酸肽抗体
速率散射比浊法
性能评价
Anti-cyclic cirullinated peptide
Rate nephelometry
Performance evaluation
