摘要
目的探讨胡桃醌对宫颈癌Caski细胞增殖影响及其促凋亡作用。方法选取处于对数生长期的宫颈癌Caski细胞,将其分为对照组和不同剂量胡桃醌组(20、40、60、80及100μmol/L)。采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法观察胡桃醌对宫颈癌Caski细胞的增殖抑制作用,并计算出半数抑制浓度(IC50),依据IC50确定胡桃醌的有效浓度;在光学显微镜下观察细胞形态学变化;Hoechst 33258染色观察细胞核形态,流式细胞术测定胡桃醌对Caski细胞凋亡影响。结果 MTT结果显示,与对照组比较,胡桃醌40、60、80、100μmol/L组宫颈癌Caski细胞增殖[OD值分别为(0.65±0.11)、(0.53±0.14)、(0.40±0.11)和(0.31±0.05)]明显受到抑制,差异均有统计学意义(P<0.05);Hoechst 33258染色显示,40μmol/L胡桃醌培养12 h可明显导致细胞核浓缩;流式细胞仪检测表明,对照组Caski细胞早期凋亡率为(2.86±0.47)%,经40μmol/L的胡桃醌处理细胞12 h,早期凋亡率增加至(15.47±2.56)%(P<0.05)。结论不同浓度的胡桃醌可以抑制Caski细胞增殖,同时可诱导Caski细胞凋亡。
Objective To explore the effects of juglone on proliferation and apoptosis of human cervical cancer Caski cells.Methods Cultured Caski cells were incubated with 20,40,60,80,and 100 μmol /L juglone for 24 hours and the untreated cells were used as the control.The proliferation of Caski cells was detected with 3-（4,5）-dimethylthiahiazo-（-z-yl）-3,5-diphenytetrazoliumromide（MTT） assay.Optical microscope was used to observe morphological changes of the cells.Nuclear fragmentation and apoptotic body were observed with Hoechst 33258 staining,and the cell apoptosis was detected with flow cytometry（FCM）.Results MTT results showed that the growth of Caski cell was greatly inhibited by 40,60,80,and 100 μmol /L juglone,with the optical density（OD） values of 0.65 ± 0.11,0.53 ± 0.14,0.40 ± 0.11,and 0.31 ± 0.05,respectively（P 〈0.05 for all） and a dose-dependent trend compared with that of the control group.Hoechst 33258 staining results showed typical morphological changes in Caski cells cultured with 40 μmol /L juglone for 12 hours.Flow cytometry results showed that the early apoptosis ratio of Caski cells was 2.86 ± 0.47 %,but the ratio of the cells treated with 40 μmol /L juglone was increased to 15.47 ± 2.56 %（P 〈0.05）.Conclusion Juglone significantly inhibits the proliferation and induces the apoptosis of Caski cells in vitro.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2014年第10期1279-1281,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(21102055)
吉林省科技厅科技发展项目(20130101157JC)
吉林省教育厅"十一五"教育技术研究项目(2009-314)
吉林省教育厅"十二五"教育技术研究项目(2011-286)
关键词
胡桃醌
细胞增殖
细胞凋亡
juglone
cell proliferation
cell apoptosis
