摘要
研究了超声辅助法提取红曲霉菌体中的红曲红、红曲黄两种红曲色素的影响因素,在单因素的实验基础上,利用响应面法优化了红曲色素的提取工艺参数,建立了二次回归模型,得到提取工艺的最佳条件。结果表明,乙醇浓度、料液比、超声功率3个因素对红曲色素的提取有显著影响,在乙醇体积分数76.30%,液料比34.44∶1,超声功率为304.84 W条件下,提取液中红色素组分色价高达5 679.4 U/g,黄色素组分色价高达4 633 U/g,与国标法提取红曲色素相比,提取率分别提高了6.5%和8.9%。
Extraction of Monascus pigments in mycelia by ultrasonic-assisted technology,on the basis of single factor experiments,using response surface method optimizes the extraction of red pigment process parameters.A quadratic regression model was established,and the optimum extraction conditions were obtained.The results showed that ethanol concentration,solid-liquid ratio,ultrasonic wave power of these three factors on the extraction of red pigment had a significant influence,ethanol concentration 76.30％,solid-liquid ratio 34.44∶1,ultrasonic wave power 304.84 W.Under the optimum conditions,the red pigment concentration of the extract reached the maximum 5 679.4 U/g,the yellow pigment was as high as 4 633 U/g.With the national standard compared to red pigment extraction,the extraction rate increased by 6.5％ and 8.9％.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2014年第8期805-813,共9页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(31301411)
北京市教育委员会科技计划面上项目(0142132014)
关键词
红曲色素
超声波
单因素实验
响应面法
Monascus pigments
ultrasonic
single factor experiments
response surface method
