摘要
目的 探讨胃黏膜活检组织中DNA含量对胃癌的早期诊断、鉴别诊断及预后判断的价值。方法 采用流式细胞仪测定 114例胃癌及各型胃炎患者胃黏膜活检组织的DNA含量 ,并与病理检查结果进行比较。结果 ①流式细胞术与病理检查结果的总符合率为 85 .96 %( 98/114 ) ,且有异倍体的非肿瘤组织可能存在较高的恶变可能性 ;②高分化肿瘤组织的DNA异倍体发生率与中分化及低分化或未分化的肿瘤相比 ,分别有显著性差异 (P <0 .0 5 )和非常显著性差异 (P <0 .0 1)。③有淋巴结转移及无淋巴结转移组DNA异倍体有非常显著性差异 (P <0 .0 1)。结论 采用流式细胞仪测定胃镜下活检胃黏膜组织的DNA含量来判断肿瘤良、恶性 ,其临床诊断与病理诊断有较高的符合率 ;在判断良性疾病癌变的可能性及预后判断上比病理诊断更有优势 ,可作为病理诊断的辅助方法应用于临床。
Objective To evaluate the clinical significance of DNA content analysis from gastroscope biopsy samples.Methods DNA content of gastroscope biopsy sample from 114 patients with gastric carcinoma and gastritis was detected by flow cytometry and compared with pathological results.Results ①The total coincidence of flow cytometry analysis and pathological result was 85.96%(98/114),histological normal tissues with aneuploid had high risk of canceration.②The incidence of DNA aneuploid in high differentiated tumor is lower than that in middle differentiated tumor(P<0.05) and low differentiated or undifferentiated tumor(P<0.01).③The incidence of DNA aneuploid in tumor with lymph node transfer was higher than that in tumor without lymph node transfer(P<0.01).Conclusion DNA content analysis by flow cytometry is not only in accord with pathological examination for the diagnosis of gastric carcinoma but also superior to pathological examination for the judgement of canceration risk of benign tissues and prognosis of malignant tumor,which should be defined as an assist method of pathological diagnosis
出处
《实用癌症杂志》
2002年第4期372-374,共3页
The Practical Journal of Cancer
