摘要
目的探讨葡萄籽原花青素(GSPE)对脑缺血再灌注大鼠学习记忆能力的影响及其机制。方法 72只健康雄性Sprague-Dawley大鼠分为假手术组(n=18)、模型组(n=18)、GSPE低剂量组(20 mg/kg,n=18)和GSPE高剂量组(200 mg/kg,n=18)。造模前,GSPE各组灌胃4周,假手术组和模型组给予蒸馏水10 ml/kg?d。线栓法制备脑缺血再灌注损伤模型。分别于缺血2 h再灌注后12 h、24 h、48 h各取6只大鼠,行Morris水迷宫测试,HE染色观察脑组织形态变化,检测大鼠脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)的含量。结果与假手术组比较,模型组Morris水迷宫测试潜伏期延长,穿台次数减少(P<0.05);HE染色显示脑组织神经元逐渐坏死;SOD含量降低,MDA含量增加(P<0.05)。与模型组比较,GSPE高剂量组各相同时间点潜伏期缩短,穿台次数增加(P<0.05);HE染色显示脑组织神经元核固缩和空泡减少;SOD含量增加,MDA含量降低(P<0.05)。结论 GSPE可以减轻脑缺血区病理改变,减轻缺血再灌注后脂质过氧化,改善脑缺血再灌注大鼠的学习记忆功能。
Objective To investigate the effect of grape seed proanthocyanidin extract （GSPE） on learning and memory ability after cerebral ischemia/reperfusion injury in rats and its mechanism. Methods 72 healthy male Sprague-Dawley rats were divided into sham group （n= 18）, model group （n=18） and GSPE groups （20 mg/kg, 200 mg/kg, n=18 for each group）. The GSPE groups were administered GSPE orally for 4 weeks, while the sham group and model group were given water 10 ml/kg. Then their middle cerebral arteries were obstructed for 2 h and reperfused, excepted the sham group. 6 rats from each group were selected to test with Morris water maze 12 h, 24 h and 48 h after reperfusion respectively. And then, their brain tissues were stained with Hematoxylin-Eosin staining to observe under optical microscope. The level of superoxide dismutase （SOD） and malondialdehyde （MDA） in the brain tissues were measured. Results Compared with the sham group, the latency significantly prolonged, and the incidence of crossing the area the platform located reduced in the model group in the Morris water maze test, with the SOD decreasing and MDA increasing （P〈0.05）. Compared with the model group, the latency reduced and the incidence of crossing the area increased in the GSPE 200 mg/kg group, with the SOD increasing and MDA decreasing （P〈0.05）. Conclusion GSPE may suppress peroxidation after the cerebral ischemia/reperfusion to protect brain and learning and memory ability from injury.
出处
《中国康复理论与实践》
CSCD
北大核心
2014年第9期827-830,共4页
Chinese Journal of Rehabilitation Theory and Practice
关键词
脑缺血/再灌注
葡萄籽原花青素
水迷宫
超氧化物歧化酶
丙二醛
大鼠
cerebral ischemia/reperfusion
grape seed proanthocyanidin extract
Morris water maze
superoxide dismutase
malondialdehyde
rats
