摘要
目的研究白芍总苷(TGP)对果糖-高脂诱导非酒精性脂肪性肝病大鼠(NAFLD)脂质浸润及纤维化的作用。方法建立果糖-高脂诱导NAFLD大鼠模型,用药组分别灌胃给予二甲双胍(200 mg·kg-1)、TGP大剂量、小剂量(给予TGP 200,100 mg·kg-1);正常对照组和模型对照组均灌胃等量纯化水(10 mL·kg-1)。4周后,各组大鼠腹主动脉取血,检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性;空腹血糖(FBG)、三酰甘油(TG)含量;检测胰岛素(INS)含量,计算胰岛素敏感指数(ISI)的变化;检测游离脂肪酸(FFA),脂肪细胞因子(Apelin-36)、内脏脂肪素(visfatin)、胶原Ⅲ(COLⅢ)及胶原Ⅳ(COLⅣ)的含量;解剖大鼠,取肝脏,测定肝组织中TG含量,光镜下观察肝脏组织病理学改变。结果与模型对照组比较,TGP能有效降低NAFLD大鼠的FBG、Fins含量,提高ISI指数;降低血清和肝组织中TG含量,降低血清ALT、AST活性及FAA、Apelin,visfatin,COLⅢ,COLⅣ含量,结果均差异有统计学意义(P<0.05或P<0.01);镜检显示TGP能改善NAFLD大鼠肝脏脂质浸润及纤维化的病理学改变。结论 TGP可能通过改善NAFLD大鼠异常的糖脂代谢及拮抗胰岛素抵抗,有效抑制NAFLD大鼠的脂质浸润及纤维化。
Objective To investigate the effect of total glucosides of paeong（TGP） on the liver lipid infiltration and fibrosis in rats with non-alcoholic fatty liver disease（NAFLD） induced by fructose and high-fat diet. Methods Fructose-highfatty induced NAFLD rat model was established. Metformin（ MET,200 mg · kg^-1） and TGP（200,100 mg·kg^-1） was intragastrically given to the rats in the treatment group,TGP high dose and low dose group,respectively. Normal control group and model control group was intragastrically treated with equivalent distilled water（10 mL· kg^-1）. At the fourth week after the treatment,all the rats were sacrificed and the indices such as serum fasting blood glucose（ FBG）,INS,insulin sensitivity index（ISI）,triglycerides（TG）,apelin-36,visfatin,alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,free fatty acid（FFA）,collagen Ⅲ（COLⅢ）,collagen Ⅳ（COLⅣ） were determined. Hepatic content of TG was determined and the pathological changes in the liver tissues were observed under the microscope. Results As compared with the model control group,TGP effectively decreased FBG,INS,TG in serum and liver tissues,activity of ALT and AST in serum and content of FAA,Apelin,Visfatin,COLⅢ and COLⅣ,with significant differences（P〈 0. 05 or P 〈0. 01）. TGP alleviated lipid infiltration and fibrosis in rat liver tissues. Conclusion TGP can inhibit effectively lipid infiltration and fibrosis of NAFLD rats,probably through improving glucolipid metabolism and antogonizing insulin resistance.
出处
《医药导报》
CAS
北大核心
2014年第10期1294-1299,共6页
Herald of Medicine
基金
广州市科技计划项目(2010Y1-C491)
广州市中医药中西医结合科技项目(2009-A-10)
关键词
白芍总苷
果糖
肝病
脂肪性
非酒精性
脂质浸润
肝纤维化
Total glucosides of paeong
Fructose
Liver disease
fatty
nonalcoholic
Lipid infiltration
Liver fibrosis
