摘要
为了发掘小麦育性基因,采用同源克隆的方法,从小麦花药中克隆了3个与拟南芥AtMS2和水稻OsMS2基因同源的cDNA序列。TaMSR-1、TaMSR-2和TaMSR-3分别具有1 842,1 824,1 830 bp的开放阅读框,各自编码613,607,609个氨基酸。推导的蛋白质序列中包含2个保守区:NAD_binding和Sterile保守功能域。氨基酸序列分析发现,TaMSR与水稻OsMSR2和拟南芥AtMSR2具有较高的相似性。采用基因特异定位引物PCR技术对中国春缺失系材料进行扩增,将TaMSR-1、TaMSR-2和TaMSR-3基因分别定位于4AS、4DL和4BL染色体上。半定量RT-PCR分析表明,TaMSR基因是花药组织特异表达的基因。这些结果说明,TaMSR基因可能在小麦花药发育过程中起重要作用。
In search for fertility gene in wheat , three cDNAs that homologous to AtMS2 and putative OsMS2 , designated as TaMSR-1 ,TaMSR-2 and TaMSR-3 were cloned from wheat anther by homologogy-based cloning ap-proach .TaMSR-1 ,TaMSR-2 and TaMSR-3 contain open reading frames of 1 842 ,1 824 ,1 830 bp encoding proteins of 613 ,607 ,609 amino acid residues including two male sterile conserved domains：NAD-binding domain and male sterile C-terminal domain , respectively .The deduced amino acid sequence of TaMSR had high similarities with OsMSR2 and AtMSR2.Using Chinese_Spring_Deletion line,TaMSR-1,TaMSR-2 and TaMSR-3 genes were located on chromosome 4AS,4DL and 4BL,respectively.Semi-quantitative RT-PCR analysis revealed that TaMSR gene was anther specific expressed gene .These results suggested that TaMSR may plays an important role in wheat anther de-velopment .
出处
《华北农学报》
CSCD
北大核心
2014年第4期32-36,共5页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31360264)
新疆维吾尔自治区自然科学基金项目(2013211B19)
新疆维吾尔自治区科技支疆项目(201191218)
