摘要
将番鸭细小病毒(MDPV)主要结构蛋白VP3基因和免疫刺激基序(CpG)依次克隆至质粒pIRES-dIL2上,构建了重组质粒pIRES-dIL2-VP3-CpG。核酸疫苗重组质粒转染BHK-21细胞进行间接免疫荧光试验,可在细胞浆中检测到绿色荧光,表明重组质粒可以成功地在真核细胞内表达。
VP3 gene of Muscovy duck parvovirus and CpG motifs were sequenced and cloned into the expression vector pIRES-dIL2.The recombinant plasmid pIRES-dIL2-VP3-CpG was constructed successfully. Then the recombinant plasmid was transfected into BHK-21 cells with LipofectamineTM2000. Green fluorescence of the transfected cells could be observed by indirect immunofluorescence test,demonstrating that expression ofthe recombinant plasmid in eukaryotic cells was successful.
出处
《中国兽医杂志》
CAS
北大核心
2014年第8期17-19,共3页
Chinese Journal of Veterinary Medicine
基金
公益性行业(农业)科研专项(201003012)
广东省农业科学院动物卫生研究所所长基金
广东省自然科学基金博士启动项目(S2011040001867)
